产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
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[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDH kinase 1; Pdk1; PDK1_HUMAN; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1;
抗原和靶标
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
- Q15118 PDK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA
翻译修饰 - Q15118 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| Y136 | Phosphorylation | P11362 (FGFR1) | Uniprot |
| K230 | Ubiquitination | Uniprot | |
| Y243 | Phosphorylation | P11362 (FGFR1) , O60674 (JAK2) , P00519 (ABL1) , P36888 (FLT3) | Uniprot |
| Y244 | Phosphorylation | P11362 (FGFR1) | Uniprot |
| R319 | Methylation | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | P00558 (PGK1) | Uniprot |
| T346 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot |
翻译修饰 - Q15118 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P05106-1 (ITGB3) | T779 | Uniprot |
| P08559 (PDHA1) | S232 | Uniprot |
| P08559 (PDHA1) | S293 | Uniprot |
| P08559 (PDHA1) | S300 | Uniprot |
| P23443-1 (RPS6KB1) | T390 | Uniprot |
| P29803 (PDHA2) | S230 | Uniprot |
| P29803 (PDHA2) | S291 | Uniprot |
| P29803 (PDHA2) | S298 | Uniprot |
| P31749-1 (AKT1) | T308 | Uniprot |
| P31749-1 (AKT1) | S473 | Uniprot |
| P31751 (AKT2) | T309 | Uniprot |
| Q15118-1 (PDK1) | S25 | Uniprot |
| Q15118-1 (PDK1) | S393 | Uniprot |
| Q15118-1 (PDK1) | S396 | Uniprot |
| Q16513 (PKN2) | T816 | Uniprot |
| Q16513-1 (PKN2) | T951 | Uniprot |
| Q9Y243-1 (AKT3) | S120 | Uniprot |
| Q9Y243 (AKT3) | T305 | Uniprot |
研究背景
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.
Mitochondrion matrix.
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).
Belongs to the PDK/BCKDK protein kinase family.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
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