产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA, E. coli, homolog of, 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serine 25; PRSS 25; PRSS25; Serine protease 25; Serine protease HTRA2; Serine protease HTRA2 mitochondrial; Serine protease htra2 mitochondrial precursor; Serine protease omi; Serine proteinase OMI;
抗原和靶标
Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
- O43464 HTRA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE
翻译修饰 - O43464 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R5 | Methylation | Uniprot | |
| R8 | Methylation | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Q9BXM7 (PINK1) , P53778 (MAPK12) | Uniprot |
| T157 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | P31751 (AKT2) , P31749 (AKT1) | Uniprot |
| K237 | Ubiquitination | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| S400 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| T453 | Phosphorylation | Uniprot |
研究背景
Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Autoproteolytically activated.
Mitochondrion intermembrane space. Mitochondrion membrane>Single-pass membrane protein.
Note: Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.
Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions. The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with BIRC6/bruce.
The mature N-terminus is involved in the interaction with XIAP.
The PDZ domain mediates interaction with MXI2.
Belongs to the peptidase S1C family.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
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