产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
BAP 1; Bap1; BAP1_HUMAN; BRCA 1 associated protein 1; BRCA1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 13; Cerebral protein 6; DKFZp686N04275; FLJ35406; FLJ37180; HUCEP 13; Hucep 6; HUCEP13; Hucep6; KIAA0272; TPDS; Ubiquitin carboxy terminal hydrolase; Ubiquitin carboxyl terminal hydrolase BAP 1; Ubiquitin carboxyl terminal hydrolase BAP1; Ubiquitin carboxyl-terminal hydrolase BAP1; UCHL2;
翻译修饰 - Q92560 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K120 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K155 | Acetylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T273 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| Y401 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| T487 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| T517 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| K656 | Methylation | Uniprot | |
| K658 | Methylation | Uniprot | |
| K659 | Methylation | Uniprot | |
| S705 | Phosphorylation | Uniprot |
研究背景
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.
Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention.
Cytoplasm. Nucleus.
Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).
Highly expressed in testis, placenta and ovary. Expressed in breast.
Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1. Interacts (when phosphorylated at Thr-493) with FOXK1. Interacts (when phosphorylated at Thr-493) with FOXK2; leading to recruit the PR-DUB complex and repress FOXK2 target genes.
Belongs to the peptidase C12 family. BAP1 subfamily.
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