产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Homolog of Latheo; homolog of latheo, Drosophila; LAT; LATHEO; LATHEO, drosophila, homolog of; orc3; ORC3_HUMAN; ORC3L; Origin recognition complex subunit 3; Origin recognition complex subunit 3 honolog; Origin Recognition Complex Subunit 3 Isoform 1; Origin recognition complex subunit 3 like; Origin recognition complex subunit Latheo; Origin Recognition Complex, subunit 3, S. cerevisiae, homolog of; Origin recognition complex, subunit 3-like (S. cerevisiae); Origin recognition complex, subunit 3-like (yeast);
抗原和靶标
- Q9UBD5 ORC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATSSMSKGCFVFKPNSKKRKISLPIEDYFNKGKNEPEDSKLRFETYQLIWQQMKSENERLQEELNKNLFDNLIEFLQKSHSGFQKNSRDLGGQIKLREIPTAALVLGVNVTDHDLTFGSLTEALQNNVTPYVVSLQAKDCPDMKHFLQKLISQLMDCCVDIKSKEEESVHVTQRKTHYSMDSLSSWYMTVTQKTDPKMLSKKRTTSSQWQSPPVVVILKDMESFATKVLQDFIIISSQHLHEFPLILIFGIATSPIIIHRLLPHAVSSLLCIELFQSLSCKEHLTTVLDKLLLTTQFPFKINEKVLQVLTNIFLYHDFSVQNFIKGLQLSLLEHFYSQPLSVLCCNLPEAKRRINFLSNNQCENIRRLPSFRRYVEKQASEKQVALLTNERYLKEETQLLLENLHVYHMNYFLVLRCLHKFTSSLPKYPLGRQIRELYCTCLEKNIWDSEEYASVLQLLRMLAKDELMTILEKCFKVFKSYCENHLGSTAKRIEEFLAQFQSLDETKEEEDASGSQPKGLQKTDLYHLQKSLLEMKELRRSKKQTKFEVLRENVVNFIDCLVREYLLPPETQPLHEVVYFSAAHALREHLNAAPRIALHTALNNPYYYLKNEALKSEEGCIPNIAPDICIAYKLHLECSRLINLVDWSEAFATVVTAAEKMDANSATSEEMNEIIHARFIRAVSELELLGFIKPTKQKTDHVARLTWGGC
翻译修饰 - Q9UBD5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K21 | Acetylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K145 | Acetylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K543 | Acetylation | Uniprot | |
| Y607 | Phosphorylation | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| K694 | Ubiquitination | Uniprot |
研究背景
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Multi-mono-ubiquitinated by OBI1; ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle.
Nucleus. Chromosome.
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.
Belongs to the ORC3 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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