产品: Caldesmon 小鼠 单克隆 抗体
货号: BF8474
描述: Mouse monoclonal antibody to Caldesmon
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog
分子量: 70~85kD,120~150kD; 93kD(Calculated).
蛋白号: Q05682

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Monoclonal [AFfirm8474]
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CAD; CALD 1; CALD1; CALD1_HUMAN; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q05682 CALD1_HUMAN:

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

描述:
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction.
序列:
MDDFERRRELRRQKREEMRLEAERIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNAQNSVPDEEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQNDTAENETTEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQVEVMVEEKTTESQEETVVMSLKNGQISSEEPKQEEEREQGSDEISHHEKMEEEDKERAEAERARLEAEERERIKAEQDKKIADERARIEAEEKAAAQERERREAEERERMREEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRARAEEEEKAKVEEQKRNKQLEEKKHAMQETKIKGEKVEQKIEGKWVNEKKAQEDKLQTAVLKKQGEEKGTKVQAKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTEVKSQNGEFMTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDDKKPFKCFTPKGSSLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTSPTKV

翻译修饰 - Q05682 作为底物

Site PTM Type Enzyme
Y27 Phosphorylation
S58 Phosphorylation
S73 Phosphorylation Q15746 (MYLK)
T83 Phosphorylation
T84 Phosphorylation
T86 Phosphorylation
K119 Ubiquitination
T124 Phosphorylation
S129 Phosphorylation
S131 Phosphorylation
T141 Phosphorylation
S153 Phosphorylation
Y160 Phosphorylation
K170 Ubiquitination
Y172 Phosphorylation
K174 Ubiquitination
K197 Sumoylation
S202 Phosphorylation
S219 Phosphorylation
S248 Phosphorylation
T402 Phosphorylation
K427 Acetylation
K479 Ubiquitination
S493 Phosphorylation
T506 Phosphorylation
T509 Phosphorylation
S511 Phosphorylation
S518 Phosphorylation
T521 Phosphorylation
K534 Acetylation
T546 Phosphorylation
S548 Phosphorylation
K569 Ubiquitination
S628 Phosphorylation
T638 Phosphorylation
K640 Acetylation
S642 Phosphorylation
S643 Phosphorylation P17252 (PRKCA) , Q9UQM7 (CAMK2A)
K645 Acetylation
K645 Sumoylation
K655 Acetylation
K655 Ubiquitination
S656 Phosphorylation P17252 (PRKCA) , Q9UQM7 (CAMK2A)
S660 Phosphorylation
S661 Phosphorylation
T666 Phosphorylation Q15746 (MYLK)
S673 Phosphorylation
K674 Ubiquitination
S677 Phosphorylation P17252 (PRKCA)
Y682 Phosphorylation
S684 Phosphorylation
K690 Ubiquitination
S691 Phosphorylation
T695 Phosphorylation
S700 Phosphorylation Q15746 (MYLK)
K713 Ubiquitination
S714 Phosphorylation O75914 (PAK3) , Q13177 (PAK2)
K718 Ubiquitination
S723 Phosphorylation
S724 Phosphorylation
T726 Phosphorylation
T730 Phosphorylation P24941 (CDK2) , Q00534 (CDK6)
K733 Acetylation
K733 Ubiquitination
T735 Phosphorylation Q15746 (MYLK)
S744 Phosphorylation Q15746 (MYLK) , Q13177 (PAK2) , O75914 (PAK3)
K752 Acetylation
T753 Phosphorylation
K758 Acetylation
S759 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S765 Phosphorylation
S773 Phosphorylation
K775 Methylation
K775 Ubiquitination
S783 Phosphorylation
T788 Phosphorylation
S789 Phosphorylation P27361 (MAPK3) , Q00534 (CDK6) , P28482 (MAPK1)
T791 Phosphorylation

研究背景

功能:

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

翻译修饰:

In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity).

细胞定位:

Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril. Cytoplasm>Cytoskeleton>Stress fiber.
Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

蛋白家族:

The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.

Belongs to the caldesmon family.

研究领域

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

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