产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
CMD1I; CSM1; CSM2; DES; DESM_HUMAN; Desmin; FLJ12025; FLJ39719; FLJ41013; FLJ41793; Intermediate filament protein; OTTHUMP00000064865;
抗原和靶标
- P17661 DESM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQAYSSSQRVSSYRRTFGGAPGFPLGSPLSSPVFPRAGFGSKGSSSSVTSRVYQVSRTSGGAGGLGSLRASRLGTTRTPSSYGAGELLDFSLADAVNQEFLTTRTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVNRLKGREPTRVAELYEEELRELRRQVEVLTNQRARVDVERDNLLDDLQRLKAKLQEEIQLKEEAENNLAAFRADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQYETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRELEDRFASEASGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL
翻译修饰 - P17661 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1) | Uniprot |
| S13 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | P17612 (PRKACA) , Q13464 (ROCK1) , Q96GD4 (AURKB) | Uniprot |
| S28 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S42 | Phosphorylation | Uniprot | |
| K43 | Methylation | Uniprot | |
| S45 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y54 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Q13464 (ROCK1) , Q96GD4 (AURKB) , P17612 (PRKACA) | Uniprot |
| S68 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Q13464 (ROCK1) , P17612 (PRKACA) | Uniprot |
| T77 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T79 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| Y281 | Phosphorylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| R315 | Methylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| Y331 | Phosphorylation | Uniprot | |
| T332 | Phosphorylation | Uniprot | |
| K339 | Acetylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| Y363 | Phosphorylation | Uniprot | |
| R386 | Methylation | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| K407 | Acetylation | Uniprot | |
| K407 | Sumoylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T450 | Phosphorylation | Uniprot |
研究背景
Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).
ADP-ribosylation prevents ability to form intermediate filaments.
Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.
Cytoplasm>Myofibril>Sarcomere>Z line. Cytoplasm. Cell membrane>Sarcolemma. Nucleus.
Note: Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190). Localizes in the nucleus exclusively in differentiating cardiac progenitor cells and premature cardiomyocytes (By similarity).
Homopolymer. Interacts with DST (By similarity). Interacts with MTM1. Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament. Interacts with CRYAB. Interacts with NEB (via nebulin repeats 160-164). Interacts (via rod region) with NEBL (via nebulin repeats 1-5).
Belongs to the intermediate filament family.
研究领域
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
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