AFfirm™ ASAP1 Mouse Monoclonal Antibody - #BF8533

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产品: ASAP1 小鼠 单克隆 抗体
货号: BF8533
描述: Mouse monoclonal antibody to ASAP1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 125kDa; 125kD(Calculated).
蛋白号: Q9ULH1

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   规格 价格 库存
 100ul RMB¥ 2300 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Monoclonal [AFfirm8533]
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein; 130 kDa phosphatidylinositol 4; 5-biphosphate-dependent ARF1 GTPase-activating protein; ADP ribosylation factor directed GTPase activating protein 1; ADP-ribosylation factor-directed GTPase-activating protein 1; AMAP 1; AMAP1; ANK repeat and PH domain-containing protein 1; ARF GTPase activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain; ASAP 1; ASAP1; ASAP1_HUMAN; Centaurin beta 4; CentB4; DDEF 1; DDEF1 protein; DEF-1; Development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; KIAA1249; PAG 2; PAG2; PAP; PIP2 dependent ARF1 GAP; PIP2-dependent ARF1 GAP; ZG 14P; ZG14P;

抗原和靶标

免疫原:
Uniprot:

Q9ULH1(ASAP1_HUMAN) >>Visit HPA database.

基因/基因ID:
ASAP1(50807)
序列:
MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKATIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

翻译修饰 - Q9ULH1 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
T34 Phosphorylation
K101 Ubiquitination
S129 Phosphorylation
K182 Acetylation
K184 Acetylation
K209 Ubiquitination
K232 Ubiquitination
K256 Ubiquitination
K276 Ubiquitination
K284 Ubiquitination
S305 Phosphorylation
Y312 Phosphorylation
T372 Phosphorylation
S433 Phosphorylation
S493 Phosphorylation
S514 Phosphorylation
S525 Phosphorylation
K555 Acetylation
K563 Acetylation
S717 Phosphorylation
S726 Phosphorylation
S733 Phosphorylation
S738 Phosphorylation
S744 Phosphorylation
S746 Phosphorylation
S757 Phosphorylation
T758 Phosphorylation
Y767 Phosphorylation Q14289 (PTK2B)
K800 Ubiquitination
S816 Phosphorylation
K824 Ubiquitination
T837 Phosphorylation
S839 Phosphorylation
S843 Phosphorylation
S865 Phosphorylation
S877 Phosphorylation
S881 Phosphorylation
K905 Acetylation
S910 Phosphorylation
T915 Phosphorylation
S924 Phosphorylation
S925 Phosphorylation
S1008 Phosphorylation
S1027 Phosphorylation
S1032 Phosphorylation
S1041 Phosphorylation
S1044 Phosphorylation
T1048 Phosphorylation
T1054 Phosphorylation
T1097 Phosphorylation
S1122 Phosphorylation
S1128 Phosphorylation

研究背景

功能:

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.

翻译修饰:

Phosphorylated on tyrosine residues by SRC.

细胞定位:

Cytoplasm. Membrane.
Note: Predominantly cytoplasmic. Partially membrane-associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2 (By similarity). Interacts with CTTN. Interacts (via SH3 domain) with APC.

蛋白家族:

The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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