AFfirm™ MAP2 Mouse Monoclonal Antibody - #BF8607

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产品: MAP2 小鼠 单克隆 抗体
货号: BF8607
描述: Mouse monoclonal antibody to MAP2
应用: WB
反应: Human
预测: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 70-80kD(C/D),250kD(A/B); 200kD(Calculated).
蛋白号: P11137

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm8607]
特异性:
MAP2 Mouse Monoclonal Antibody detects endogenous levels of total MAP2.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp686I2148; MAP 2; MAP dendrite specific; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; MTAP2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P11137(MTAP2_HUMAN) >>Visit HPA database.

基因/基因ID:
MAP2(4133)
描述:
Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).
序列:
MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTYPDKKDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKDEWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFLQPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEAINQETVQQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSEQKDQEPTTDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGAATSAELDMPFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQTGKESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLGGRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCFPVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSETVVEDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFYEGTDDKVRRDLATDLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEKSEEHADSKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVEQGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTKVSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEIPCPPAVSEADLATDERADVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQGEYDKLLFRSDTLQITDLGVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRFAALEQPEVERRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDDSIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKEPSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL

翻译修饰 - P11137 作为底物

Site PTM Type Enzyme
Y50 Phosphorylation
S63 Phosphorylation
T66 Phosphorylation
Y67 Phosphorylation P07948 (LYN) , P06241 (FYN)
S136 Phosphorylation
T168 Phosphorylation
T216 Phosphorylation
S233 Phosphorylation
T241 Phosphorylation
T258 Phosphorylation
S285 Phosphorylation
T290 Phosphorylation
S311 Phosphorylation
S315 Phosphorylation
S321 Phosphorylation
T323 Phosphorylation
S357 Phosphorylation
T361 Phosphorylation
S385 Phosphorylation Q9P0L2 (MARK1)
T427 Phosphorylation
T516 Phosphorylation
S532 Phosphorylation
S585 Phosphorylation
Y592 Phosphorylation
Y593 Phosphorylation
S610 Phosphorylation
S629 Phosphorylation
S725 Phosphorylation
S785 Phosphorylation
S788 Phosphorylation
S821 Phosphorylation
S823 Phosphorylation
S833 Phosphorylation
S843 Phosphorylation
T860 Phosphorylation
S862 Phosphorylation
S881 Phosphorylation
S913 Phosphorylation
Y996 Phosphorylation
S1005 Phosphorylation
S1021 Phosphorylation
S1031 Phosphorylation
S1106 Phosphorylation
T1108 Phosphorylation
S1139 Phosphorylation
T1154 Phosphorylation
S1155 Phosphorylation
S1158 Phosphorylation
S1234 Phosphorylation
S1324 Phosphorylation
S1347 Phosphorylation
S1353 Phosphorylation
T1366 Phosphorylation
T1368 Phosphorylation
Y1369 Phosphorylation
S1398 Phosphorylation
S1420 Phosphorylation
S1421 Phosphorylation
K1427 Acetylation
T1440 Phosphorylation
K1447 Ubiquitination
K1448 Ubiquitination
S1480 Phosphorylation
Y1493 Phosphorylation
T1494 Phosphorylation
T1497 Phosphorylation
S1500 Phosphorylation
K1505 Acetylation
T1506 Phosphorylation
K1524 Acetylation
S1534 Phosphorylation
S1539 Phosphorylation
S1540 Phosphorylation
S1546 Phosphorylation
S1555 Phosphorylation
S1562 Phosphorylation
S1564 Phosphorylation
S1588 Phosphorylation
T1590 Phosphorylation
S1591 Phosphorylation
T1592 Phosphorylation
T1594 Phosphorylation
T1595 Phosphorylation
S1598 Phosphorylation
T1599 Phosphorylation
T1602 Phosphorylation
T1605 Phosphorylation
S1608 Phosphorylation
Y1609 Phosphorylation
S1611 Phosphorylation
T1613 Phosphorylation
T1616 Phosphorylation
T1619 Phosphorylation
S1621 Phosphorylation
Y1622 Phosphorylation
T1628 Phosphorylation
T1631 Phosphorylation
S1634 Phosphorylation
S1640 Phosphorylation
T1649 Phosphorylation
S1653 Phosphorylation
T1656 Phosphorylation
S1675 Phosphorylation
S1679 Phosphorylation P17612 (PRKACA) , Q9P0L2 (MARK1)
T1680 Phosphorylation
S1702 Phosphorylation
S1706 Phosphorylation
S1710 Phosphorylation Q9P0L2 (MARK1) , P17612 (PRKACA)
S1727 Phosphorylation
S1742 Phosphorylation P17612 (PRKACA)
T1780 Phosphorylation
S1782 Phosphorylation P17612 (PRKACA)
S1786 Phosphorylation
S1787 Phosphorylation
S1790 Phosphorylation
S1795 Phosphorylation
S1798 Phosphorylation
S1799 Phosphorylation
S1800 Phosphorylation
S1802 Phosphorylation
S1808 Phosphorylation
T1813 Phosphorylation
T1819 Phosphorylation

研究背景

功能:

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

翻译修饰:

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).

细胞定位:

Cytoplasm>Cytoskeleton. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites.

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