产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte cytosolic protein 2; SH2 domain containing leukocyte protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein;
抗原和靶标
A synthesized peptide derived from human SLP76.
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
- Q13094 LCP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRNVPFRSEVLGWDPDSLADYFKKLNYKDCEKAVKKYHIDGARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGEDDGDYESPNEEEEAPVEDDADYEPPPSNDEEALQNSILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP
翻译修饰 - Q13094 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y29 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK) | Uniprot |
| Y128 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK) | Uniprot |
| S130 | Phosphorylation | Uniprot | |
| Y145 | Phosphorylation | P43403 (ZAP70) , P06239 (LCK) , P42681 (TXK) | Uniprot |
| S169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y173 | Phosphorylation | Q08881 (ITK) | Uniprot |
| S207 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| R273 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| R392 | Methylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
| S425 | Phosphorylation | Uniprot | |
| Y426 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
| T428 | Phosphorylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| T454 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| Y465 | Phosphorylation | Uniprot | |
| Y470 | Phosphorylation | Uniprot | |
| Y476 | Phosphorylation | Uniprot | |
| Y483 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| Y522 | Phosphorylation | Uniprot | |
| Y532 | Phosphorylation | Uniprot |
研究背景
Involved in T-cell antigen receptor mediated signaling.
Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.
Cytoplasm.
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Interacts with SLA. Interacts with CBLB (By similarity). Interacts with GRB2. Interacts with SHB. Interacts with PRAM1. Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus). Interacts with FYB1 and the phosphorylated form of FYB2.
The SH2 domain mediates interaction with phosphorylated CD6 (PubMed:16914752). The SH2 domain mediates interaction with SHB (PubMed:12084069).
研究领域
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
限制条款
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