产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
130 kDa retinoblastoma associated protein; 130 kDa retinoblastoma-associated protein; IB4; p130; PPAR-alpha-interacting complex protein 128; PRB2; Rb2; Rbl2; RBL2_HUMAN; RBR 2; RBR-2; RBR2; Retinoblastoma like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2; RIC128;
抗原和靶标
- Q08999 RBL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFFRKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH
翻译修饰 - Q08999 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S98 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| T401 | Phosphorylation | P11802 (CDK4) , Q00534 (CDK6) | Uniprot |
| T404 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T417 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K443 | Ubiquitination | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| Y544 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T642 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T647 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
| T661 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T664 | Phosphorylation | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| Y667 | Phosphorylation | Uniprot | |
| Y670 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Q00534 (CDK6) , P11802 (CDK4) | Uniprot |
| S676 | Phosphorylation | Uniprot | |
| T678 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S690 | Phosphorylation | Uniprot | |
| T694 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S941 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S952 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T954 | Phosphorylation | Uniprot | |
| T961 | Phosphorylation | Uniprot | |
| S962 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| S973 | Phosphorylation | Uniprot | |
| T974 | Phosphorylation | Uniprot | |
| S981 | Phosphorylation | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| T986 | Phosphorylation | Uniprot | |
| T988 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| T1019 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | P11802 (CDK4) , Q00534 (CDK6) | Uniprot |
| Y1037 | Phosphorylation | Uniprot | |
| T1042 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | P24941 (CDK2) | Uniprot |
| Y1057 | Phosphorylation | Uniprot | |
| S1059 | Phosphorylation | Uniprot | |
| T1065 | Phosphorylation | Uniprot | |
| S1068 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1078 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1082 | Phosphorylation | Uniprot | |
| S1090 | Phosphorylation | Uniprot | |
| T1097 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1112 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1138 | Phosphorylation | Uniprot |
研究背景
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
Nucleus.
Interacts with AATF. Interacts with KMT5B, KMT5C and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with RBBP9 (By similarity).
Belongs to the retinoblastoma protein (RB) family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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