产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN;
抗原和靶标
- P60842 IF4A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
翻译修饰 - P60842 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Methylation | Uniprot | |
| K54 | Sumoylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| C66 | S-Nitrosylation | Uniprot | |
| K68 | Sumoylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K146 | Sumoylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| R168 | Methylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Methylation | Uniprot | |
| K193 | Sumoylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| K202 | Sumoylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K225 | Sumoylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Sumoylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| R255 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K291 | Sumoylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K309 | Acetylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| R316 | Methylation | Uniprot | |
| R353 | Methylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K381 | Acetylation | Uniprot | |
| K381 | Ubiquitination | Uniprot |
研究背景
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.
Belongs to the DEAD box helicase family. eIF4A subfamily.
研究领域
· Genetic Information Processing > Translation > RNA transport.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.