产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP 1; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; Ptpn6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1; SHP1L; tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6;
抗原和靶标
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
- P29350 PTN6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK
翻译修饰 - P29350 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| K277 | Acetylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| R286 | Methylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| C453 | S-Nitrosylation | Uniprot | |
| K486 | Ubiquitination | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| Y509 | Phosphorylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| Y536 | Phosphorylation | P06213 (INSR) , P00519 (ABL1) , P12931 (SRC) , A0A173G4P4 (Abl fusion) , P06239 (LCK) , P07948 (LYN) | Uniprot |
| T540 | Phosphorylation | Uniprot | |
| Y541 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| Y564 | Phosphorylation | A0A173G4P4 (Abl fusion) , P00519 (ABL1) , P07948 (LYN) , P06239 (LCK) , P12931 (SRC) | Uniprot |
| K570 | Ubiquitination | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | P17252 (PRKCA) , P24941 (CDK2) | Uniprot |
研究背景
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.
Cytoplasm. Nucleus.
Note: In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Interacts with KIT (By similarity). Interacts with SIRPA/PTPNS1. Interacts with LILRB1 and LILRB2. Interacts with FCRL2 and FCRL4. Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form). Interacts with CD84. Interacts with CD300LF. Interacts with CDK2. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with moesin/MSN.
The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
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