产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Cytosine 5methyltransferase 3B; DNA; DNA (cytosine 5) methyltransferase 3 beta; DNA (cytosine 5)-methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; DNM3B_HUMAN; Dnmt3b; EC 2.1.1.37; ICF; ICF1; M.HsaIIIB; MGC124407; RP23-89H14.3;
抗原和靶标
Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
- Q9UBC3 DNM3B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDESPVEFPATRSLRRRATASAGTPWPSPPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGMESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDGKFSEVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVRAGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE
翻译修饰 - Q9UBC3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S51 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| T105 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| Y404 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| S711 | Phosphorylation | Uniprot | |
| K777 | Ubiquitination | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| Y815 | Phosphorylation | Uniprot | |
| T816 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| R823 | Methylation | Uniprot | |
| R826 | Methylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| K847 | Ubiquitination | Uniprot |
研究背景
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.
Sumoylated.
Citrullinated by PADI4.
Nucleus.
Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex.
The PWWP domain is essential for targeting to pericentric heterochromatin.
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
研究领域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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