AFfirm™ Hexokinase 1 Mouse Monoclonal Antibody - #BF9305

产品: Hexokinase 1 小鼠 单克隆 抗体
货号: BF9305
描述: Mouse monoclonal antibody to Hexokinase 1
应用: WB
反应: Human
预测: Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 109 KD; 102kD(Calculated).
蛋白号: P19367

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm9305]
特异性:
Hexokinase 1 Mouse Monoclonal Antibody detects endogenous levels of total Hexokinase 1.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Brain form hexokinase; Hexokinase 1; Hexokinase type I; HK I; HK1 ta; HK1 tb; HK1 tc; HKI; HXK1;

抗原和靶标

免疫原:
Uniprot:

P19367(HXK1_HUMAN) >>Visit HPA database.

基因/基因ID:
HK1(3098)
表达:
P19367 HXK1_HUMAN:

Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502).

序列:
MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS

翻译修饰 - P19367 作为底物

Site PTM Type Enzyme
M1 Acetylation
Y25 Phosphorylation
Y27 Phosphorylation
K62 Ubiquitination
K77 Acetylation
S124 Phosphorylation
K147 Ubiquitination
S155 Phosphorylation
K173 Ubiquitination
K176 Methylation
K176 Ubiquitination
S178 Phosphorylation
K187 Ubiquitination
K191 Ubiquitination
S269 Phosphorylation
K290 Ubiquitination
S298 Phosphorylation
Y301 Phosphorylation
K315 Ubiquitination
K333 Ubiquitination
S337 Phosphorylation
S340 Phosphorylation
K344 Ubiquitination
K346 Ubiquitination
S364 Phosphorylation
T403 Phosphorylation
Y417 Phosphorylation
K418 Ubiquitination
T419 Phosphorylation
Y461 Phosphorylation
K489 Acetylation
K501 Ubiquitination
T503 Phosphorylation
K510 Ubiquitination
K544 Acetylation
K624 Ubiquitination
Y686 Phosphorylation
R727 Methylation
Y732 Phosphorylation P12931 (SRC)
S733 Phosphorylation
K738 Ubiquitination
Y749 Phosphorylation
K763 Acetylation
K763 Ubiquitination
K777 Ubiquitination
S792 Phosphorylation
C813 S-Nitrosylation
T821 Phosphorylation
C823 S-Nitrosylation
S827 Phosphorylation
R849 Methylation
K880 Ubiquitination
K885 Ubiquitination
S893 Phosphorylation
T913 Phosphorylation

研究背景

功能:

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.

细胞定位:

Mitochondrion outer membrane>Peripheral membrane protein. Cytoplasm>Cytosol.
Note: The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.

亚基结构:

Monomer. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2. Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity).

蛋白家族:

The N- and C-terminal halves of this hexokinase contain a hexokinase domain (PubMed:9493266, PubMed:9735292, PubMed:10574795). The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus (PubMed:9493266, PubMed:9735292, PubMed:10574795). Each domain can bind a single D-glucose and D-glucose 6-phosphate molecule (PubMed:9493266).

Belongs to the hexokinase family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Biosynthesis of other secondary metabolites > Neomycin, kanamycin and gentamicin biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

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