AFfirm™ AATF Mouse Monoclonal Antibody - #BF9325
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
AATF; AATF_HUMAN; Apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; BFR2; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Rb-binding protein Che-1;
抗原和靶标
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
- Q9NY61 AATF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPQPLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQEELLFQYPDTRYLVDGTKPNAGSEEISSEDDELVEEKKQQRRRVPAKRKLEMEDYPSFMAKRFADFTVYRNRTLQKWHDKTKLASGKLGKGFGAFERSILTQIDHILMDKERLLRRTQTKRSVYRVLGKPEPAAQPVPESLPGEPEILPQAPANAHLKDLDEEIFDDDDFYHQLLRELIERKTSSLDPNDQVAMGRQWLAIQKLRSKIHKKVDRKASKGRKLRFHVLSKLLSFMAPIDHTTMNDDARTELYRSLFGQLHPPDEGHGD
翻译修饰 - Q9NY61 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K58 | Sumoylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S143 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K145 | Ubiquitination | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S203 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| K223 | Sumoylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K276 | Methylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K342 | Sumoylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| T366 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| K369 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K475 | Sumoylation | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| T476 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K496 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | O96017 (CHEK2) | Uniprot |
研究背景
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Hyperphosphorylated during the G1/S phase transition.
Nucleus>Nucleolus.
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.
Belongs to the AATF family.
限制条款
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