AFfirm™ p73 Mouse Monoclonal Antibody - #BF9326
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Delta N p73; DNp73; p53 like transcription factor; p53 related protein; P73; TP73;
抗原和靶标
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
- O15350 P73_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH
翻译修饰 - O15350 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T14 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | P53350 (PLK1) | Uniprot |
| Y28 | Phosphorylation | P08631 (HCK) | Uniprot |
| S47 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S48 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| T86 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| Y99 | Phosphorylation | P00519 (ABL1) , A0A173G4P4 (Abl fusion) | Uniprot |
| T167 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | O14965 (AURKA) | Uniprot |
| S289 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K321 | Acetylation | Uniprot | |
| K327 | Acetylation | Uniprot | |
| K331 | Acetylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | P05771-2 (PRKCB) , P17252 (PRKCA) | Uniprot |
| S426 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S471 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| K627 | Sumoylation | Uniprot |
研究背景
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.
Nucleus. Cytoplasm.
Note: Accumulates in the nucleus in response to DNA damage.
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation.
(Microbial infection) Interacts with Epstein-Barr virus protein EBNA6; this interaction inhibits TP73-mediated apoptotic pathway.
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL1 tyrosine kinase SH3 domain.
The PPxY motif mediates interaction with WWOX.
Belongs to the p53 family.
研究领域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Measles.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
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