AFfirm™ MAP4K4 Mouse Monoclonal Antibody - #BF9334

产品: MAP4K4 小鼠 单克隆 抗体
货号: BF9334
描述: Mouse monoclonal antibody to MAP4K4
应用: WB
反应: Human
预测: Mouse, Rat, Pig, Bovine, Horse, Rabbit, Dog
分子量: 142kDa; 142kD(Calculated).
蛋白号: O95819

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm9334]
特异性:
MAP4K4 Mouse Monoclonal Antibody detects endogenous levels of total MAP4K4.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLH21957; FLJ10410; FLJ20373; FLJ90111; Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; HPK/GCK-like kinase HGK; KIAA0687; M4K4_HUMAN; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; Nck-interacting kinase; NIK;

抗原和靶标

免疫原:
Uniprot:

O95819(M4K4_HUMAN) >>Visit HPA database.

基因/基因ID:
MAP4K4(9448)
表达:
O95819 M4K4_HUMAN:

Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

描述:
a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.
序列:
MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

翻译修饰 - O95819 作为底物

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
S17 Phosphorylation
T35 Phosphorylation
Y36 Phosphorylation
K46 Ubiquitination
K54 Ubiquitination
T59 Phosphorylation
Y86 Phosphorylation
S93 Phosphorylation
T124 Phosphorylation
Y132 Phosphorylation
S134 Phosphorylation
K155 Ubiquitination
S175 Phosphorylation
T181 Phosphorylation
T187 Phosphorylation
T191 Phosphorylation
Y193 Phosphorylation
K261 Ubiquitination
S264 Phosphorylation
K285 Ubiquitination
K303 Ubiquitination
T319 Phosphorylation
Y321 Phosphorylation
Y323 Phosphorylation
S324 Phosphorylation
S326 Phosphorylation
T351 Phosphorylation
Y467 Phosphorylation
Y520 Phosphorylation
S523 Phosphorylation
S536 Phosphorylation
R546 Methylation
S547 Phosphorylation
K548 Ubiquitination
S550 Phosphorylation
Y560 Phosphorylation
T576 Phosphorylation
S580 Phosphorylation
S598 Phosphorylation
S600 Phosphorylation
S602 Phosphorylation
S604 Phosphorylation
T627 Phosphorylation
T628 Phosphorylation
S629 Phosphorylation
S631 Phosphorylation
S639 Phosphorylation
S644 Phosphorylation
S649 Phosphorylation
S656 Phosphorylation
S677 Phosphorylation
S679 Phosphorylation
S680 Phosphorylation
S681 Phosphorylation
S683 Phosphorylation
S684 Phosphorylation
S686 Phosphorylation
S688 Phosphorylation
S692 Phosphorylation
S696 Phosphorylation
S698 Phosphorylation
S700 Phosphorylation
S708 Phosphorylation
S709 Phosphorylation
S710 Phosphorylation
S712 Phosphorylation
S715 Phosphorylation
S717 Phosphorylation
K738 Ubiquitination
T743 Phosphorylation
S791 Phosphorylation
S800 Phosphorylation
S801 Phosphorylation
S805 Phosphorylation
T809 Phosphorylation
S811 Phosphorylation
S823 Phosphorylation
T828 Phosphorylation
S830 Phosphorylation
K831 Ubiquitination
T834 Phosphorylation
T840 Phosphorylation
S842 Phosphorylation
T846 Phosphorylation
K849 Methylation
K849 Ubiquitination
K851 Methylation
K851 Ubiquitination
S852 Phosphorylation
S853 Phosphorylation
S854 Phosphorylation
S855 Phosphorylation
T857 Phosphorylation
S868 Phosphorylation
S870 Phosphorylation
S871 Phosphorylation
T873 Phosphorylation
T874 Phosphorylation
T876 Phosphorylation
S877 Phosphorylation
K898 Ubiquitination
S900 Phosphorylation
T907 Phosphorylation
S913 Phosphorylation
T915 Phosphorylation
K957 Ubiquitination
Y992 Phosphorylation
S995 Phosphorylation
K1010 Ubiquitination
K1053 Ubiquitination
K1057 Ubiquitination
K1070 Ubiquitination
Y1143 Phosphorylation
T1146 Phosphorylation
K1152 Ubiquitination
K1179 Ubiquitination
K1198 Ubiquitination
K1204 Ubiquitination
K1212 Ubiquitination
S1224 Phosphorylation
Y1227 Phosphorylation
T1230 Phosphorylation

翻译修饰 - O95819 作为激酶

Substrate Site Source
P15311 (EZR) T567 Uniprot
P26038 (MSN) T558 Uniprot
P35241 (RDX) T564 Uniprot

研究背景

功能:

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

亚基结构:

Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially).

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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