AFfirm™ USP13 Mouse Monoclonal Antibody - #BF9358
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
Deubiquitinating enzyme 13; Isopeptidase T 3; Isopeptidase T-3; Isopeptidase T3; ISOT 3; ISOT-3; ISOT3; Ubiquitin carboxyl terminal hydrolase 13; Ubiquitin carboxyl-terminal hydrolase 13; Ubiquitin specific peptidase 13 (isopeptidase T3); Ubiquitin specific peptidase 13; Ubiquitin specific processing protease 13; Ubiquitin specific protease 13; Ubiquitin thioesterase 13; Ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13; UBP13; UBP13_HUMAN; USP 13; USP13;
抗原和靶标
- Q92995 UBP13_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLAFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLDTDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLWLNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVLDPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS
翻译修饰 - Q92995 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R4 | Methylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| R101 | Methylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K405 | Sumoylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K511 | Ubiquitination | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K598 | Ubiquitination | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| K640 | Sumoylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| K841 | Ubiquitination | Uniprot | |
| K850 | Ubiquitination | Uniprot |
研究背景
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Highly expressed in ovary and testes.
Interacts with UFD1. Interacts (via UBA domains) with SIAH2 (when ubiquitinated). Interacts with BAG6; the interaction is direct and may mediate UBL4A deubiquitination. Interacts (via UBA 2 domain) with AMFR; the interaction is direct. Interacts with UBL4A; may be indirect via BAG6.
The UBP-type zinc finger has lost its ability to bind ubiquitin and USP13 is not activated by unanchored ubiquitin. Swapping with the UBP-type zinc finger from USP5 restores ability to bind unanchored ubiquitin and subsequent activation of the protein (PubMed:22216260).
The UBA domains mediate binding to ubiquitin.
Belongs to the peptidase C19 family.
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