AFfirm™ NAMPT Mouse Monoclonal Antibody - #BF9373
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
1110035O14Rik; AI314458; AI480535; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nampt; NAMPT_HUMAN; Nicotinamide phosphoribosyltransferase; PBEF 1; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; VF; Visfatin;
抗原和靶标
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
- P43490 NAMPT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH
翻译修饰 - P43490 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| Y18 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| Y26 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| Y87 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| Y188 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| R196 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K369 | Acetylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K415 | Acetylation | Uniprot | |
| K423 | Acetylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot |
研究背景
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
Nucleus. Cytoplasm. Secreted.
Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum.
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Homodimer.
Belongs to the NAPRTase family.
研究领域
· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
限制条款
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