产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
3 hydroxy 3 methylglutaryl CoA reductase; 3 hydroxy 3 methylglutaryl Coenzyme A reductase; 3 hydroxymethylglutaryl CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3H3M; HMDH_HUMAN; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; Hmgcr; Hydroxymethylglutaryl CoA reductase; LDLCQ3; MGC103269; Red;
抗原和靶标
- P04035 HMDH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASTLAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTADTSKVSLGLDENVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLALLLAVKYIFFEQTETESTLSLKNPITSPVVTQKKVPDNCCRREPMLVRNNQKCDSVEEETGINRERKVEVIKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTA
翻译修饰 - P04035 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K89 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| T431 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K502 | Ubiquitination | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| Y644 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K666 | Ubiquitination | Uniprot | |
| K670 | Ubiquitination | Uniprot | |
| K692 | Sumoylation | Uniprot | |
| K692 | Ubiquitination | Uniprot | |
| K704 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K864 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| K873 | Ubiquitination | Uniprot | |
| T884 | Phosphorylation | Uniprot | |
| K885 | Ubiquitination | Uniprot |
研究背景
Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner.
Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Homodimer. Interacts (via its SSD) with INSIG1; the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with UBIAD1.
Belongs to the HMG-CoA reductase family.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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