AFfirm™ TLR1 Mouse Monoclonal Antibody - #BF9397

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产品: TLR1 小鼠 单克隆 抗体
货号: BF9397
描述: Mouse monoclonal antibody to TLR1
应用: WB
反应: Human
预测: Mouse, Rat, Bovine, Horse, Rabbit, Dog
分子量: 90kDa; 90kD(Calculated).
蛋白号: Q15399

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 100ul RMB¥ 1150 2300 现货

货期: 当天发货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm9397]
特异性:
TLR1 Mouse Monoclonal Antibody detects endogenous levels of total TLR1.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD281; CD281 antigen; KIAA0012; LPRS5; rsc786; TIL; TIL. LPRS5; Tlr1; TLR1_HUMAN; Toll (Drosophila) homolog; Toll like receptor 1; Toll-like receptor 1; Toll/interleukin-1 receptor-like protein;

抗原和靶标

免疫原:
Uniprot:

Q15399(TLR1_HUMAN) >>Visit HPA database.

基因/基因ID:
TLR1(7096)
表达:
Q15399 TLR1_HUMAN:

Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

描述:
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. Toll-like receptor expression is highest in peripheral blood leukocytes, monocytes, macrophages, though TLR1 expression may be less restricted than other family members (4,5). TLR1 associates with TLR2 to cooperatively mediate immune responses to bacterial lipoproteins and lead to NF-κB activation (6,7). TLR1 shows highest homology to TLR6, which shares 69% sequence identity (8).
序列:
MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSKLRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQLKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSVKTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISNVKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPFLHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCSWTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFSSLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPESYRGTLLKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRARNIPLEELQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVENIITCIEKSYKSIFVLSPNFVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIPSSYHKLKSLMARRTYLEWPKEKSKRGLFWANLRAAINIKLTEQAKK

翻译修饰 - Q15399 作为底物

Site PTM Type Enzyme
S44 Phosphorylation
T48 Phosphorylation
N51 N-Glycosylation
S53 Phosphorylation
Y56 Phosphorylation
N163 N-Glycosylation
N330 N-Glycosylation
K416 Methylation
S420 Phosphorylation
T422 Phosphorylation
N429 N-Glycosylation
S431 Phosphorylation
T529 Phosphorylation
K537 Ubiquitination
S678 Phosphorylation
T685 Phosphorylation
S690 Phosphorylation
Y691 Phosphorylation

研究背景

功能:

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

细胞定位:

Cell membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Phagosome membrane>Single-pass type I membrane protein. Membrane raft. Golgi apparatus.
Note: Does not reside in lipid rafts before stimulation but accumulates increasingly in the raft upon the presence of the microbial ligand. In response to triacylated lipoproteins, TLR2:TLR1 heterodimers are recruited in lipid rafts, this recruitment determine the intracellular targeting to the Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

亚基结构:

Interacts (via extracellular domain) with TLR2. TLR2 seems to exist in heterodimers with either TLR1 or TLR6 before stimulation by the ligand. The heterodimers form bigger oligomers in response to their corresponding ligands as well as further heterotypic associations with other receptors such as CD14 and/or CD36. The activation cluster TLR2:TLR1:CD14 forms in response to triacylated lipopeptides. Binds MYD88 (via TIR domain). Interacts with CNPY3 (By similarity).

蛋白家族:

The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Belongs to the Toll-like receptor family.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

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