Phospho-eIF4B (Ser422) Antibody - #AF5428

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产品: 磷酸化 eIF4B (Ser422) 抗体
货号: AF5428
描述: Rabbit polyclonal antibody to Phospho-eIF4B (Ser422)
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 69 kDa; 69kD(Calculated).
蛋白号: P23588
RRID: AB_2837912

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-eIF4B (Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.
RRID:
AB_2837912
引用格式: Affinity Biosciences Cat# AF5428, RRID:AB_2837912.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2310046H11Rik; AL024095; C85189; EIF 4B; eIF-4B; EIF4B; Eukaryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; IF4B_HUMAN; PRO1843;

抗原和靶标

免疫原:
Uniprot:

P23588(IF4B_HUMAN) >>Visit HPA database.

基因/基因ID:
EIF4B(1975)
描述:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
序列:
MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23588 作为底物

Site PTM Type Enzyme
K6 Acetylation
S16 Phosphorylation
Y33 Phosphorylation
S39 Phosphorylation
S52 Phosphorylation
S57 Phosphorylation
Y63 Phosphorylation
R70 Methylation
S71 Phosphorylation
T75 Phosphorylation
R78 Methylation
K92 Ubiquitination
S93 Phosphorylation
Y96 Phosphorylation
T97 Phosphorylation
Y105 Phosphorylation
S111 Phosphorylation
R117 Methylation
S131 Phosphorylation
Y141 Phosphorylation
S155 Phosphorylation
S160 Phosphorylation
K177 Ubiquitination
R182 Methylation
S183 Phosphorylation
S192 Phosphorylation
T195 Phosphorylation
T197 Phosphorylation
T205 Phosphorylation
S207 Phosphorylation
Y211 Phosphorylation
S219 Phosphorylation
K223 Ubiquitination
Y224 Phosphorylation
Y228 Phosphorylation
S230 Phosphorylation
Y233 Phosphorylation
Y237 Phosphorylation
Y241 Phosphorylation
Y252 Phosphorylation
Y258 Phosphorylation
R261 Methylation
S263 Phosphorylation
Y266 Phosphorylation
R268 Methylation
Y270 Phosphorylation
S272 Phosphorylation
R273 Methylation
R279 Methylation
S283 Phosphorylation
Y285 Phosphorylation
Y291 Phosphorylation
R292 Methylation
R297 Methylation
Y298 Phosphorylation
Y302 Phosphorylation
R308 Methylation
S309 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
Y316 Phosphorylation
S317 Phosphorylation
Y321 Phosphorylation
K333 Sumoylation
S340 Phosphorylation
T341 Phosphorylation
S347 Phosphorylation
S348 Phosphorylation
S350 Phosphorylation
T351 Phosphorylation
S352 Phosphorylation
S354 Phosphorylation
S359 Phosphorylation
K365 Acetylation
K365 Ubiquitination
T369 Phosphorylation
S406 Phosphorylation Q9P1W9 (PIM2) , Q14680 (MELK)
S409 Phosphorylation
T412 Phosphorylation
S418 Phosphorylation
T420 Phosphorylation
S422 Phosphorylation P51812 (RPS6KA3) , Q15418 (RPS6KA1) , P23443 (RPS6KB1) , P31749 (AKT1)
S424 Phosphorylation
S425 Phosphorylation
T427 Phosphorylation
T429 Phosphorylation
S430 Phosphorylation
T431 Phosphorylation
T432 Phosphorylation
S433 Phosphorylation
S434 Phosphorylation
S442 Phosphorylation
K444 Acetylation
S445 Phosphorylation
T450 Phosphorylation
S459 Phosphorylation
T461 Phosphorylation
S462 Phosphorylation
K463 Ubiquitination
K486 Methylation
S488 Phosphorylation
S489 Phosphorylation
S495 Phosphorylation
S497 Phosphorylation
S498 Phosphorylation
T500 Phosphorylation
S504 Phosphorylation
T506 Phosphorylation
S518 Phosphorylation
K537 Ubiquitination
S585 Phosphorylation
K586 Acetylation
K586 Ubiquitination
S589 Phosphorylation
S591 Phosphorylation
K592 Ubiquitination
Y593 Phosphorylation
S597 Phosphorylation
Y609 Phosphorylation

研究背景

功能:

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

翻译修饰:

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

亚基结构:

Self-associates and interacts with EIF3 p170 subunit.

研究领域

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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