| 产品: | 磷酸化 hnRNP K (Ser284) 抗体 |
| 货号: | AF5442 |
| 描述: | Rabbit polyclonal antibody to Phospho-hnRNP K (Ser284) |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 51 kDa; 51kD(Calculated). |
| 蛋白号: | P61978 |
| RRID: | AB_2837926 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5442, RRID:AB_2837926.
展开/折叠
CSBP; dC stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; HNRNPK; HNRPK; HNRPK_HUMAN; Transformation up regulated nuclear protein; Transformation up-regulated nuclear protein; Transformation upregulated nuclear protein; TUNP;
抗原和靶标
- P61978 HNRPK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61978 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| R22 | Methylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K34 | Methylation | Uniprot | |
| K34 | Sumoylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| R46 | Methylation | Uniprot | |
| K52 | Sumoylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K60 | Acetylation | Uniprot | |
| K60 | Sumoylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y72 | Phosphorylation | P12931 (SRC) | Uniprot |
| S75 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| Y135 | Phosphorylation | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| K139 | Methylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| R148 | Methylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K163 | Sumoylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| C184 | S-Nitrosylation | Uniprot | |
| C185 | S-Nitrosylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| R191 | Methylation | Uniprot | |
| K198 | Methylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| R201 | Methylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | P53779 (MAPK10) , P06493 (CDK1) , P24941 (CDK2) , P45983 (MAPK8) | Uniprot |
| K219 | Acetylation | Uniprot | |
| K219 | Sumoylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| Y225 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y230 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y234 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y236 | Phosphorylation | P12931 (SRC) | Uniprot |
| R246 | Methylation | Uniprot | |
| R247 | Methylation | Uniprot | |
| R249 | Methylation | Uniprot | |
| R256 | Methylation | Uniprot | |
| R258 | Methylation | Uniprot | |
| R263 | Methylation | Uniprot | |
| R268 | Methylation | Uniprot | |
| R271 | Methylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| R277 | Methylation | Uniprot | |
| R278 | Methylation | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| R286 | Methylation | Uniprot | |
| R296 | Methylation | Uniprot | |
| R299 | Methylation | Uniprot | |
| S302 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| R303 | Methylation | Uniprot | |
| R305 | Methylation | Uniprot | |
| R316 | Methylation | Uniprot | |
| Y323 | Phosphorylation | Uniprot | |
| R325 | Methylation | Uniprot | |
| R326 | Methylation | Uniprot | |
| R328 | Methylation | Uniprot | |
| Y333 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | P53779 (MAPK10) , P45983 (MAPK8) , P27361 (MAPK3) | Uniprot |
| Y361 | Phosphorylation | Uniprot | |
| Y369 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| Y373 | Phosphorylation | Uniprot | |
| R377 | Methylation | Uniprot | |
| S379 | Phosphorylation | O14965 (AURKA) | Uniprot |
| Y380 | Phosphorylation | P12931 (SRC) | Uniprot |
| T389 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| K405 | Sumoylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| R433 | Methylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| Y449 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| Y458 | Phosphorylation | P12931 (SRC) | Uniprot |
| K461 | Acetylation | Uniprot | |
| K461 | Ubiquitination | Uniprot |
研究背景
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.
Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cytoplasm. Nucleus>Nucleoplasm. Cell projection>Podosome.
Note: Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).
Interacts with RBM42 and ZIK1 (By similarity). Interacts with BRDT (By similarity). Identified in the spliceosome C complex. Interacts with ANKRD28. Interacts with ASFV p30 protein. Interacts with DDX1. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation. Interacts with p53/TP53. Interacts with IVNS1ABP.
(Microbial infection) Interacts with HCV core protein.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
限制条款
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