Collagen II Antibody - #AF5456

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产品: Collagen II 抗体
货号: AF5456
描述: Rabbit polyclonal antibody to Collagen II
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 141 kDa; 142kD(Calculated).
蛋白号: P02458
RRID: AB_2837940

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(80%), Xenopus(90%)
克隆:
Polyclonal
特异性:
Collagen II Antibody detects endogenous levels of total Collagen II.
RRID:
AB_2837940
引用格式: Affinity Biosciences Cat# AF5456, RRID:AB_2837940.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha 1 type II collagen;Alpha-1 type II collagen;AOM;Cartilage collagen;Chondrocalcin;CO2A1_HUMAN;COL11A3;Col2a1;Collagen II alpha 1 polypeptide;SEDC;col 2;

抗原和靶标

免疫原:
Uniprot:

P02458(CO2A1_HUMAN) >>Visit HPA database.

基因/基因ID:
COL2A1(1280)
表达:
P02458 CO2A1_HUMAN:

Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

描述:
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
序列:
MIRLGAPQTLVLLTLLVAAVLRCQGQDVQEAGSCVQDGQRYNDKDVWKPEPCRICVCDTGTVLCDDIICEDVKDCLSPEIPFGECCPICPTDLATASGQPGPKGQKGEPGDIKDIVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQLGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKAGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGEPGPAGPQGAPGPAGEEGKRGARGEPGGVGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGASGPAGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPAGSAGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGEQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPPGPPGPSGKDGPKGARGDSGPPGRAGEPGLQGPAGPPGEKGEPGDDGPSGAEGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGAVGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIQGPQGPRGDKGEAGEPGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGANGIPGPIGPPGPRGRSGETGPAGPPGNPGPPGPPGPPGPGIDMSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
90
Chicken
80
Dog
56
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02458 作为底物

Site PTM Type Enzyme
Y41 Phosphorylation
K490 Ubiquitination
S536 Phosphorylation
K542 Acetylation
K764 Acetylation
K773 Acetylation
T1307 Phosphorylation
S1416 Phosphorylation

研究背景

功能:

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

翻译修饰:

The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.

Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains.

Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.

Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.

O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

亚基结构:

Homotrimers of alpha 1(II) chains.

蛋白家族:

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity).

Belongs to the fibrillar collagen family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE, 2022 (PubMed: 35905566) [IF=7.9]
2). Vitamin K2 ameliorates osteoarthritis by suppressing ferroptosis and extracellular matrix degradation through activation GPX4's dual functions. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38759289) [IF=7.5]

Application: WB    Species: Rat    Sample:

Fig. 8. GPX4 inhibitor RSL3 substantially attenuates VK2 protection of chondrocytes. (A-I) WB results for GPX4, NFκB, pMAPK/MAPK, Aggrecan, CollagenⅡ, SOX9, MMP3, MMP13 and Tubulin; (J) Quantitive analysis of relative Glutathione Peroxidase Activity; (K-L) GSH contents and ratio of GSH/GSSG; (M) Quantitative results of MDA content assay.
3). Oleic and linoleic acids promote chondrocyte apoptosis by inhibiting autophagy via downregulation of SIRT1/FOXO1 signaling. Biochimica et biophysica acta. Molecular basis of disease, 2024 (PubMed: 38378085) [IF=6.2]

Application: WB    Species: Rat    Sample:

Fig. 6. Regulation of the expression of extracellular matrix components in chondrocytes by SIRT1 and FOXO1. (A-E) Chondrocytes were transfected with siRNAs against FOXO1 and SIRT1 or with FOXO1- and SIRT1-expressing adenoviruses. (A) Western blot analysis of ADAMTS5, collagen II, and collagen X expression. (B) Quantitative analysis of the protein levels in (A) (n = 3). (C) Real-time PCR analysis of ADAMTS5, collagen II, and collagen X expression. (D) Confocal images of collagen II in chondrocytes (scale bar, 50 μm). (E) Fluorescence intensities of collagen II in (D). Error bars present mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Application: IF/ICC    Species: Rat    Sample:

Fig. 6. Regulation of the expression of extracellular matrix components in chondrocytes by SIRT1 and FOXO1. (A-E) Chondrocytes were transfected with siRNAs against FOXO1 and SIRT1 or with FOXO1- and SIRT1-expressing adenoviruses. (A) Western blot analysis of ADAMTS5, collagen II, and collagen X expression. (B) Quantitative analysis of the protein levels in (A) (n = 3). (C) Real-time PCR analysis of ADAMTS5, collagen II, and collagen X expression. (D) Confocal images of collagen II in chondrocytes (scale bar, 50 μm). (E) Fluorescence intensities of collagen II in (D). Error bars present mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001.
4). Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells. Experimental and Therapeutic Medicine, 2020 (PubMed: 32256772) [IF=2.7]

Application: WB    Species: Human    Sample: SW1353 cells

Figure 2 Effect of irisin on MMP-13 and Col-II in IL-1β-induced SW1353 cells. SW1353 cells were treated with 20 mM irisin for 24 h, with or without 10 ng/ml IL-1β. (A) Col II protein levels, (B) MMP-13 protein levels, (C) Col II and MMP-13 protein expression, (D) Col II mRNA levels and (E) MMP-13 mRNA levels were evaluated by reverse transcription-quantitative PCR and western blot analysis (n=5/group). *P<0.05, **P<0.01 and ***P<0.001. IL-1β, interleukin-1β; MMP-13, matrix metalloproteinase-13; Col-II, collagen II; NC, negative control.

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