(40)
(9)
产品描述
来源:
Mouse
应用:
WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:200, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
All
克隆:
Monoclonal [T177]
RRID:
AB_2839412
引用格式: Affinity Biosciences Cat# T0003, RRID:AB_2839412.
引用格式: Affinity Biosciences Cat# T0003, RRID:AB_2839412.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG tag antibody
抗原和靶标
免疫原:
A synthetic peptide DYKDDDDK coupled to KLH.
描述:
FLAG tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.
文献引用
1). Young LINE-1 transposon 5′ UTRs marked by elongation factor ELL3 function as enhancers to regulate naïve pluripotency in embryonic stem cells. Nature Cell Biology, 2023
(PubMed: 37591949)
[IF=17.3]
2). An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP. The Journal of clinical investigation, 2022
(PubMed: 34905508)
[IF=13.3]
3). Ultrasound Guided Local Delivery of Bioorthogonal PDL1 Degrader for Enhanced Immunotherapy. Small (Weinheim an der Bergstrasse, Germany), 2025
(PubMed: 39511869)
[IF=13.0]
4). Psychologic Stress Drives Progression of Malignant Tumors via DRD2/HIF1α Signaling. CANCER RESEARCH, 2021
(PubMed: 34321238)
[IF=12.5]
Application: WB Species: mouse Sample:
Fig. 4 |DRD2 overexpression inhibited the ubiquitin-dependent degradation of HIF1α. N, Effect of DRD2 on the content of ubiquitin binding to HIF1α. DRD2-overexpressing cells treated with MG132, and cellular extracts were prepared for co-IP assay with anti–HIF1α antibody, followed by Western blotting detection of ubiquitin. siRNA, small interfering RNA. ns, nonsignificant; *, P < 0.05; **, P < 0.01
5). Nucleolar protein PEXF controls ribosomal RNA synthesis and pluripotency exit. Developmental cell, 2025
(PubMed: 39729985)
[IF=10.7]
6). Origin of the OAS-RNase L innate immune pathway before the rise of jawed vertebrates via molecular tinkering. Proceedings of the National Academy of Sciences of the United States of America, 2023
(PubMed: 37487089)
[IF=9.4]
7). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019
(PubMed: 31669149)
[IF=9.1]
8). Voluntary exercise alleviates neural functional deficits in Parkinson's disease mice by inhibiting microglial ferroptosis via SLC7A11/ALOX12 axis. NPJ Parkinson's disease, 2025
(PubMed: 40122927)
[IF=8.7]
9). WWP2 drives the progression of gastric cancer by facilitating the ubiquitination and degradation of LATS1 protein. Cell Communication and Signaling, 2023
(PubMed: 36803368)
[IF=8.4]
10). SPINK13 acts as a tumor suppressor in hepatocellular carcinoma by inhibiting Akt phosphorylation. Cell death & disease, 2024
(PubMed: 39537605)
[IF=8.1]
限制条款
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