产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0021, RRID:AB_2834083.
展开/折叠
Cdc 25a; CDC25A; CDC25A2; CDC25A2 CAG isoform; Cell division cycle 25 homolog A (S. pombe); Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform b; D9Ertd393e; Dual specificity phosphatase Cdc25A; M phase inducer phosphatase 1; M-phase inducer phosphatase 1; MGC115549; MPIP1_HUMAN;
抗原和靶标
- P30304 MPIP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELGPEPPHRRRLLFACSPPPASQPVVKALFGASAAGGLSPVTNLTVTMDQLQGLGSDYEQPLEVKNNSNLQRMGSSESTDSGFCLDSPGPLDSKENLENPMRRIHSLPQKLLGCSPALKRSHSDSLDHDIFQLIDPDENKENEAFEFKKPVRPVSRGCLHSHGLQEGKDLFTQRQNSAPARMLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLASSPKGTIENILDNDPRDLIGDFSKGYLFHTVAGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKKPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFMKCQSYCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30304 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S18 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S40 | Phosphorylation | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | O14757 (CHEK1) , P49841 (GSK3B) | Uniprot |
| S77 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Q8NG66 (NEK11) , P48729 (CSNK1A1) | Uniprot |
| T80 | Phosphorylation | Q9H4B4 (PLK3) | Uniprot |
| S82 | Phosphorylation | P48729 (CSNK1A1) , Q8NG66 (NEK11) | Uniprot |
| S88 | Phosphorylation | Q8NG66 (NEK11) | Uniprot |
| S107 | Phosphorylation | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K120 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Q683Z8 (CHK2) , O96017 (CHEK2) , O14757 (CHEK1) | Uniprot |
| S126 | Phosphorylation | Uniprot | |
| K150 | Acetylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Q683Z8 (CHK2) , O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| K257 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | P24941 (CDK2) | Uniprot |
| R278 | Methylation | Uniprot | |
| S279 | Phosphorylation | Q683Z8 (CHK2) , O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| S283 | Phosphorylation | Uniprot | |
| R290 | Methylation | Uniprot | |
| S293 | Phosphorylation | O14757 (CHEK1) , O96017 (CHEK2) , Q15418 (RPS6KA1) , Q683Z8 (CHK2) | Uniprot |
| S295 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| K300 | Ubiquitination | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| Y486 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S513 | Phosphorylation | Q9H4B4 (PLK3) | Uniprot |
| Y518 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Q9H4B4 (PLK3) | Uniprot |
研究背景
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
Interacts with CCNB1/cyclin B1. Interacts with YWHAE/14-3-3 epsilon when phosphorylated. Interacts with CUL1 specifically when CUL1 is neddylated and active. Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage. Interacts with PIM1. Interacts with CHEK2; mediates CDC25A phosphorylation and degradation in response to infrared-induced DNA damages. Interacts with HSP90AB1; prevents heat shock-mediated CDC25A degradation and contributes to cell cycle progression.
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
Belongs to the MPI phosphatase family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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