Phospho-Gab2 (Tyr452) Antibody - #AF0059

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产品: 磷酸化 Gab2 (Tyr452) 抗体
货号: AF0059
描述: Rabbit polyclonal antibody to Phospho-Gab2 (Tyr452)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 75kDa; 74kD(Calculated).
蛋白号: Q9UQC2
RRID: AB_2834102

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Xenopus(86%)
克隆:
Polyclonal
特异性:
Phospho-Gab2 (Tyr452) Antibody detects endogenous levels of Gab2 only when phosphorylated at Tyrosine 452.
RRID:
AB_2834102
引用格式: Affinity Biosciences Cat# AF0059, RRID:AB_2834102.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GAB 2; Gab2; GAB2_HUMAN; Grb 2 associated binder 2; GRB 2 associated binding protein 2; Grb2 associated binder 2; GRB2 associated binder 2 pp100; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2 associated protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; p97; PH domain containing adaptor molecule p97; pp100;

抗原和靶标

免疫原:
Uniprot:

Q9UQC2(GAB2_HUMAN) >>Visit HPA database.

基因/基因ID:
GAB2(9846)
描述:
This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
序列:
MSGGGDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNDHSKKPLRIINLNFCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEEDMNKWVQSICQICGFNQAEESTDSLRNVSSAGHGPRSSPAELSSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSNHMQPTLSTSAPQEYLYLHQCISRRAENARSASFSQGTRASFLMRSDTAVQKLAQGNGHCVNGISGQVHGFYSLPKPSRHNTEFRDSTYDLPRSLASHGHTKGSLTGSETDNEDVYTFKTPSNTLCREFGDLLVDNMDVPATPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPPRPPKPSQAETPRWGSPQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPQRGGESAGRSAESMSDGVGSFLPGKMIVGRSDSTNSEDNYVPMNPGSSTLLAMERAGDNSQSVYIPMSPGAHHFDSLGYPSTTLPVHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPITKSWSRANHTFNSSSSQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPSSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
86
Zebrafish
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UQC2 作为底物

Site PTM Type Enzyme
Phosphorylation
S2 Phosphorylation
Y48 Phosphorylation
Y49 Phosphorylation
K56 Acetylation
S133 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
S146 Phosphorylation
S147 Phosphorylation
S148 Phosphorylation
S149 Phosphorylation
S159 Phosphorylation P31749 (AKT1)
S164 Phosphorylation
T171 Phosphorylation
S177 Phosphorylation
Y194 Phosphorylation
S208 Phosphorylation
S210 Phosphorylation
S218 Phosphorylation
S223 Phosphorylation
Y249 Phosphorylation
S250 Phosphorylation
S264 Phosphorylation
T265 Phosphorylation
Y266 Phosphorylation
T278 Phosphorylation
S281 Phosphorylation
S285 Phosphorylation
T287 Phosphorylation
Y293 Phosphorylation
T294 Phosphorylation
T319 Phosphorylation
S322 Phosphorylation
Y324 Phosphorylation
T331 Phosphorylation
S368 Phosphorylation
T385 Phosphorylation
T391 Phosphorylation
S404 Phosphorylation
S405 Phosphorylation
T408 Phosphorylation
Y409 Phosphorylation
Y411 Phosphorylation
S422 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
S448 Phosphorylation
Y452 Phosphorylation P08631 (HCK)
S459 Phosphorylation
S472 Phosphorylation
S474 Phosphorylation
Y476 Phosphorylation
S480 Phosphorylation
S488 Phosphorylation
Y491 Phosphorylation
K542 Methylation
S543 Phosphorylation
T546 Phosphorylation
S550 Phosphorylation
Y563 Phosphorylation
Y584 Phosphorylation
S611 Phosphorylation
Y614 Phosphorylation P43403 (ZAP70)
S622 Phosphorylation
S623 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S625 Phosphorylation
S631 Phosphorylation
T632 Phosphorylation
S633 Phosphorylation
S634 Phosphorylation
T636 Phosphorylation
S637 Phosphorylation
Y643 Phosphorylation O60674 (JAK2)
T651 Phosphorylation
T657 Phosphorylation

研究背景

功能:

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

翻译修饰:

Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

Dephosphorylated by PTPN11.

细胞定位:

Cytoplasm. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with SHC1; may mediate interaction with receptors (By similarity). Interacts with SYK (By similarity). Interacts with PI-3 kinase. Interacts with GRB2 (via SH3 2 domain). Interacts (phosphorylated) with PTPN11. Interacts with TNFRSF11A (via cytoplasmic domain). Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling. Interacts with HCK.

蛋白家族:

The SH3-binding motifs mediate interaction with SHC1 and GRB2.

The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.

Belongs to the GAB family.

研究领域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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