产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0061, RRID:AB_2834104.
展开/折叠
MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPK5_HUMAN; MAPKAP kinase 5; MAPKAPK-5; MAPKAPK5; Mitogen activated protein kinase activated protein kinase 5; p38-regulated/activated protein kinase; PRAK;
抗原和靶标
- Q8IW41 MAPK5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ
翻译修饰 - Q8IW41 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S93 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K123 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | P53778 (MAPK12) , P28482 (MAPK1) , O15264 (MAPK13) , P31152 (MAPK4) , P45984 (MAPK9) , Q15759 (MAPK11) , Q16539 (MAPK14) , Q16659 (MAPK6) | Uniprot |
| T186 | Phosphorylation | Uniprot | |
| Y188 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot |
翻译修饰 - Q8IW41 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S377 | Uniprot |
| O43524 (FOXO3) | S215 | Uniprot |
| O60229 (KALRN) | S487 | Uniprot |
| P04637 (TP53) | S37 | Uniprot |
| P04792 (HSPB1) | S15 | Uniprot |
| P04792 (HSPB1) | S78 | Uniprot |
| P04792 (HSPB1) | S82 | Uniprot |
| P07101-3 (TH) | S19 | Uniprot |
| P07101-3 (TH) | S40 | Uniprot |
| P07101-3 (TH) | S404 | Uniprot |
| P25685 (DNAJB1) | S149 | Uniprot |
| P25685 (DNAJB1) | S151 | Uniprot |
| P25685 (DNAJB1) | S171 | Uniprot |
| P31152 (MAPK4) | S186 | Uniprot |
| P47712 (PLA2G4A) | S727 | Uniprot |
| Q15382 (RHEB) | S130 | Uniprot |
| Q92599 (SEPT8) | S163 | Uniprot |
| Q92599 (SEPT8) | S192 | Uniprot |
研究背景
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).
Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.
Expressed ubiquitously.
Interacts with ERK3/MAPK6 and ERK4/MAPK4 (via FRIEDE motif); the interaction is direct (By similarity). Interacts with YWHAE; the interaction prevents phosphorylation of HSP27/HSPB1 leading to disrupt F-actin polymerization. Interacts with SQSTM1.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.