Substance P Antibody - #DF7522

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产品: Substance P 抗体
货号: DF7522
描述: Rabbit polyclonal antibody to Substance P
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 16kDa; 15kD(Calculated).
蛋白号: P20366
RRID: AB_2841021

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
Substance P Antibody detects endogenous levels of total Substance P.
RRID:
AB_2841021
引用格式: Affinity Biosciences Cat# DF7522, RRID:AB_2841021.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C-terminal-flanking peptide; neurokinin A; neurokinin alpha; NKA; NKNA; PPT; preprotachykinin; protachykinin; protachykinin-1; SubstanceP; TAC1; TAC2; Tachykinin 1; Tachykinin precursor 1; Tachykinin1; TKN1_HUMAN; SP;

抗原和靶标

免疫原:
Uniprot:

P20366(TKN1_HUMAN) >>Visit HPA database.

基因/基因ID:
TAC1(6863)
序列:
MKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRPKPQQFFGLMGKRDADSSIEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYERRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
63
Zebrafish
63
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

翻译修饰:

The substance P form is cleaved at Pro-59 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the tachykinin family.

文献引用

1). Terrestrosin D ameliorates skin lesions in an imiquimod-induced psoriasis-like murine model by inhibiting the interaction between Substance P and Dendritic cells. PHYTOMEDICINE, 2022 (PubMed: 34923236) [IF=6.7]
2). Astragalus Polysaccharides Ameliorate Diabetic Bladder Dysfunction via Normalization of Neuromuscular Conduction. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2025 [IF=5.7]
3). Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease. Frontiers in Immunology, 2023 (PubMed: 37033943) [IF=5.7]
4). The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System–Interstitial Cells of Cajal–Smooth Muscle Cells Network. Journal of Inflammation Research, 2021 (PubMed: 34552344) [IF=4.5]
5). Zhiqiao Gancao decoction regulated JAK2/STAT3/ macrophage M1 polarization to ameliorate intervertebral disc degeneration. Heliyon, 2024 (PubMed: 39170327) [IF=4.0]

Application: WB    Species: Rat    Sample:

Fig. 2 The impact of ZQGCD on IDD rats. (A) Paw withdrawal threshold in rats. (B) Paw withdrawal latency in rats. (C) Relative expression level of substance P protein in intervertebral disc tissue of rats. The unedited images are referenced in Fig. S1 (D) Representative HE-stained intervertebral disc sections (magnification: 10 × or 40 × ). (E) Intervertebral disc histopathologic score. *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001, ns: P>0.05.
6). Clinical and Animal Studies of Waist and Knee Scraping Therapy for Knee Osteoarthritis. Combinatorial chemistry & high throughput screening, 2024 (PubMed: 38284729) [IF=1.6]
7). Electroacupuncture Modulates 5-HT4R-Mediated cAMP/PKA Signaling to Improve Intestinal Motility Disorders in a Thy1- α Syn Parkinson's Mouse Model. Evidence-based complementary and alternative medicine : eCAM, 2022 (PubMed: 36337584)

Application: WB    Species: Mouse    Sample: colon tissue

Figure 4 Effects of electroacupuncture on the expression of SP and CGRP neurotransmitters in the colon tissue of Thy1-αSyn transgenic mice. (a) mRNA expression of SP. (b) mRNA expression of CGRP. (c) Representative western blot images showing the protein expression levels of SP and CGRP. (d) Protein expression in the mouse colon tissue of the normal control (N), PD model (M), electroacupuncture (EA), and western medicine (WM) groups. ∗P < 0.05 and ∗∗P < 0.01 relative to the normal control group; #P < 0.05 and ##P < 0.01 relative to the PD model group.
8). Acupuncture Needling, Electroacupuncture, and Fire Needling Improve Imiquimod-Induced Psoriasis-Like Skin Lesions through Reducing Local Inflammatory Responses. Evidence-based Complementary and Alternative Medicine, 2019 (PubMed: 31467575)

Application: WB    Species: mouse    Sample: skin

Figure 5: | Acupuncture needling and electroacupuncture reduced the expression levels of Neurokinin A (NKA) in IMQ-induced mouse skin lesions. Te expression levels of (a) NKA, and (b) Substance P (SP) were detected by Western immunoblotting. Data are presented as the mean ± SD (n = 7/group). ##p<0.01, ###p<0.001 vs Ctrl; ∗p <0.05, ∗∗p <0.01 vs model.

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