PINK1 Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	PINK1 抗体
货号:	DF7742
描述:	Rabbit polyclonal antibody to PINK1
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Dog
分子量:	50-70kDa; 63kD(Calculated).
蛋白号:	Q9BXM7
RRID:	AB_2841209

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(89%), Bovine(83%), Horse(94%), Sheep(89%), Dog(94%)

克隆:

Polyclonal

特异性:

PINK1 Antibody detects endogenous levels of total PINK1.

RRID:

AB_2841209
引用格式: Affinity Biosciences Cat# DF7742, RRID:AB_2841209.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

BRPK; FLJ27236; mitochondrial; PARK 6; PARK6; Phosphatase and Tensin Homolog; PINK 1; PINK1; PINK1_HUMAN; Protein kinase BRPK; PTEN induced putative kinase 1; PTEN induced putative kinase protein 1; PTEN-induced putative kinase protein 1; Serine/threonine kinase PINK1 mitochondrial; Serine/threonine protein kinase PINK1 mitochondrial; Serine/threonine-protein kinase PINK1;

抗原和靶标

免疫原:

Uniprot:

Q9BXM7(PINK1_HUMAN) >>Visit HPA database.

基因/基因ID:

PINK1(65018)

表达:

Q9BXM7 PINK1_HUMAN:

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

序列:

Q9BXM7 PINK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

Dog

Pig

Sheep

Bovine

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BXM7 作为底物

Q9BXM7

Site	PTM Type	Enzyme	Source
K137	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
K186	Ubiquitination		Uniprot
S228	Phosphorylation	Q9BXM7 (PINK1)	Uniprot
S229	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
T257	Phosphorylation	Q9BXM7 (PINK1)	Uniprot
K262	Acetylation		Uniprot
S284	Phosphorylation		Uniprot
T313	Phosphorylation	Q7KZI7 (MARK2)	Uniprot
K319	Ubiquitination		Uniprot
S402	Phosphorylation	Q9BXM7 (PINK1)	Uniprot
K433	Ubiquitination		Uniprot
S465	Phosphorylation		Uniprot
S495	Phosphorylation		Uniprot
K547	Ubiquitination		Uniprot

翻译修饰 - Q9BXM7 作为激酶

Q9BXM7

Substrate	Site	Source
O15379 (HDAC3)	S424	Uniprot
O43464 (HTRA2)	S142	Uniprot
O60260 (PRKN)	S65	Uniprot
P0CG48 (UBC)	S65	Uniprot
Q8IXI2 (RHOT1)	S156	Uniprot
Q9BXM7 (PINK1)	S228	Uniprot
Q9BXM7 (PINK1)	T257	Uniprot
Q9BXM7 (PINK1)	S402	Uniprot
Q9Y2N7 (HIF3A)	T12	Uniprot

研究背景

Q9BXM7_PINK1_HUMAN

功能:

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN. Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity).

翻译修饰:

Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.

Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Processed into a 52 kDa mature form by PARL or AFG3L2 following the cleavage by mitochondrial-processing peptidase (MPP) during mitochondrial import.

细胞定位:

Mitochondrion outer membrane>Single-pass membrane protein. Mitochondrion inner membrane>Single-pass membrane protein. Cytoplasm>Cytosol.
Note: Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol.

组织特异性:

亚基结构:

Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. Interacts with NENF.

蛋白家族:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

研究领域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

文献引用

1). TRPM2 protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction via modulating autophagy. Theranostics, 2023 (PubMed: 37649595) [IF=12.4]

Application: WB Species: Mouse Sample: kidney

Figure 9 TRPM2-mediated Ca2+ influx is involved in the inhibition of the AKT-mTOR pathway in response to cisplatin. Intracellular Ca2+ signals indicated by the fluorescence of Fluo-4 AM in cisplatin (CP, 20 μM) or normal saline (NS) treated Trpm2-/- and WT MEFs (A, B) and mRTECs (C, D) (n = 7). Scale bars, 20 μm and 10μm, respectively. (E) Immunoblotting analysis and quantification of LC3B II in WT MEFs after incubation with clotrimazole (CLT, 10 μM) or vehicle (Veh) for 1 h followed by treatment with CP at the indicated concentration for 24 h. (F) Immunoblotting analysis and quantification of LC3B II in WT MEFs after incubation with 5 μM BAPTA-AM for 1 h followed by treatment with 20 μM CP for 24 h. (G) Immunoblotting analysis and quantification of p-AKT, AKT and p-p70S6K in WT MEFs after incubation with 5 μM BAPTA-AM or 10 μM clotrimazole for 1 h followed by treatment with 20 μM CP for 24 h. (H, I) Mitochondrial Ca2+ signals indicated by the fluorescence of Rhod-2 in mRTECs treated by CP or NS (n = 7). Scale bars, 10 μm. (J) Immunoblotting analysis and quantification of BNIP3 and PINK1 in mRTECs treated by CP or NS. Data are presented as mean±SEM. Statistical analysis was performed using one-way ANOVA with Tukey post-hoc test. ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001.

2). Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma. Metabolism: clinical and experimental, 2024 (PubMed: 38184165) [IF=9.8]

3). Integrating network analysis and experimental validation to reveal the mitophagy-associated mechanism of Yiqi Huoxue (YQHX) prescription in the treatment of myocardial ischemia/reperfusion injury. PHARMACOLOGICAL RESEARCH, 2023 (PubMed: 36736970) [IF=9.3]

4). Activation of aldehyde dehydrogenase-2 improves ischemic random skin flap survival in rats. Frontiers in Immunology, 2023 (PubMed: 37441072) [IF=7.3]

Application: IHC Species: Mouse Sample:

Figure 9 (A) Immunohistochemistry images of ALDH2, PINK1 and Parkin. All images were obtained at identical magnification, ×200, scale bar = 50 μm. (B) Quantitative analysis of ALDH2, PINK1 and Parkin content (n = 3). Data are represented as mean ± SEM. **P

5). Polystyrene microplastics induce mitochondrial damage in mouse GC-2 cells. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35489138) [IF=6.8]

Application: WB Species: mouse Sample: GC-2 cells

Fig. 2.| Effects of PS-MPS on mitochondrial membrane protein and membrane potential in control group and PS-MPS group after 24 h. (A) Relative transcription of mRNA of PINK1; (B) Relative protein expression of PINK1

6). 3-MCPD Induces Renal Cell Pyroptosis and Inflammation by Inhibiting ESCRT-III-Mediated Cell Repair and Mitophagy. Journal of agricultural and food chemistry, 2024 (PubMed: 38857427) [IF=6.1]

7). Walnut-Derived Peptide Enhances Mitophagy via JNK-Mediated PINK1 Activation to Reduce Oxidative Stress in HT-22 Cells. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022 (PubMed: 35187930) [IF=6.1]

8). 1, 3-Dichloro-2-propanol-Induced Renal Tubular Cell Necroptosis through the ROS/RIPK3/MLKL Pathway. Journal of Agricultural and Food Chemistry, 2022 (PubMed: 36000575) [IF=6.1]

9). Effect of Thyroxine on the Structural and Dynamic Features of Cardiac Mitochondria and Mitophagy in Rats. Cells, 2023 (PubMed: 36766738) [IF=6.0]

10). 25-Hydroxycholesterol mitigates hepatic ischemia reperfusion injury via mediating mitophagy. International Immunopharmacology, 2021 (PubMed: 33878616) [IF=5.6]

Application: WB Species: Rat Sample: liver tissues

Fig. 3. 25HC pretreatment is associated with PINK1/Parkin dependent mitophagy and NLRP3 inflammasome inhibition during hepatic I/R injury. (A) Western blotting bands and statistical analysis of mitophagy related protein P62, Parkin, PINK1, TOMM20 and LC3B. n = 4 per group. (B) The western blotting bands and statistical analysis of NLRP3, Pro-caspase1, Caspase1, Pro-IL-1β and IL-1β-P17 proteins. n = 4 per group. (C) The relative mRNA expression of proinflammatory factors TNFα, IL-6 and IL- 1β. n = 4 per group. (D)The levels of proinflammatory factors TNFα, IL-6 and IL-1β in serum. n = 4 per group. ns not significant, * p < 0.05, ** p < 0.01 and *** p < 0.001vs Sham group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IR3h + V group; & p < 0.05, && p < 0.01 and &&& p < 0.001 vs IR24h + V group. n = 3 independent experiments.

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PINK1 Antibody - #DF7742

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