APOA2 Antibody | Affinity Biosciences LTD|亲科生物官网

IF/ICC
Cites

1/3

FIGURE 1 Characterization of exosomes (EXOs).

FIGURE 1 | Characterization of exosomes (EXOs).

产品:	APOA2 抗体
货号:	DF7905
描述:	Rabbit polyclonal antibody to APOA2
应用:	WB IF/ICC
反应:	Human, Mouse, Rat
预测:	Bovine, Sheep, Dog
分子量:	11 kDa; 11kD(Calculated).
蛋白号:	P02652
RRID:	AB_2841322

浏览相似产品>>

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Bovine(80%), Sheep(80%), Dog(83%)

克隆:

Polyclonal

特异性:

APOA2 Antibody detects endogenous levels of total APOA2.

RRID:

AB_2841322
引用格式: Affinity Biosciences Cat# DF7905, RRID:AB_2841322.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

APO A2; Apo AII; Apo-AII; APOA 2; ApoA II; ApoA-II; APOA2; APOA2_HUMAN; APOAII; Apolipoprotein A II; Apolipoprotein A-II(1-76); Apolipoprotein A2; Apolipoprotein AII; ApolipoproteinA II; OTTHUMP00000032244;

抗原和靶标

免疫原:

Uniprot:

P02652(APOA2_HUMAN) >>Visit HPA database.

基因/基因ID:

APOA2(336)

表达:

P02652 APOA2_HUMAN:

Plasma; synthesized in the liver and intestine.

序列:

P02652 APOA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLLAATVLLLTICSLEGALVRRQAKEPCVESLVSQYFQTVTDYGKDLMEKVKSPELQAEAKSYFEKSKEQLTPLIKKAGTELVNFLSYFVELGTQPATQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Dog

Bovine

Sheep

Rabbit

Horse

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02652 作为底物

P02652

Site	PTM Type	Source
S32	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
Y37	Phosphorylation	Uniprot
T42	O-Glycosylation	Uniprot
S54	Phosphorylation	Uniprot
K67	Acetylation	Uniprot
S68	Phosphorylation	Uniprot

研究背景

P02652_APOA2_HUMAN

功能:

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

翻译修饰:

Phosphorylation sites are present in the extracellular medium.

Apolipoprotein A-II is O-glycosylated.

细胞定位:

Secreted.

组织特异性:

Plasma; synthesized in the liver and intestine.

亚基结构:

Monomer. Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD.Interacts with APOA1BP and NDRG1.

(Microbial infection) Interacts with HCV core protein.

蛋白家族:

Belongs to the apolipoprotein A2 family.

研究领域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). Neural stem cell-derived exosomes regulate neural stem cell differentiation through miR-9-Hes1 axis. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34055767) [IF=5.5]

Application: WB Species: Mouse Sample: neural stem cells (NSC)

FIGURE 1 Characterization of exosomes (EXOs). (A) Purified exosomes were observed under transmission electron microscopy (TEM) using negative staining. (B) The levels of positive exosomal markers TSG101, CD9, and Flotillin-1 in protein lysates of neural stem cells (NSC)-derived exosome pellets were determined by western blotting. (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting. (D,E) Particle-size distribution of exosomes was determined by NanoSight analysis (NTA) in panel (D) and NanoFCM in panel (E) technologies. Scale bar 200 nm in panel (A).

Application: WB Species: mouse Sample: NSCs

FIGURE 1 | Characterization of exosomes (EXOs). (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting.

2). Neural stem cell-derived exosomal FTO protects neuron from microglial inflammatory injury by inhibiting microglia NRF2 mRNA m6A modification. Journal of neurogenetics, 2023 (PubMed: 37812019) [IF=1.9]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.

APOA2 Antibody - #DF7905

产品推荐

相关下载

MS Report

HPLC Report

MSDS

说明书

COA