| 产品: | PSMD14 抗体 |
| 货号: | DF8011 |
| 描述: | Rabbit polyclonal antibody to PSMD14 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 35 kDa; 35kD(Calculated). |
| 蛋白号: | O00487 |
| RRID: | AB_2841387 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8011, RRID:AB_2841387.
展开/折叠
26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1; 26S proteasome-associated PAD1 homolog; PAD1; PAD1, yeast, homolog of; POH1; Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14; Proteasome 26S subunit non ATPase 14; PSDE_HUMAN; Psmd14; RPN11; Testis tissue sperm binding protein Li 69n;
抗原和靶标
- O00487 PSDE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDTVVFK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00487 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| C238 | S-Nitrosylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K277 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.
Widely expressed. Highest levels in heart and skeletal muscle.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD4, a base containing 6 ATPases and few additional components. Within the complex, PSMD4 interacts with subunit PSMD7 through their respective MPN domain. Interacts with TXNL1.
Belongs to the peptidase M67A family. PSMD14 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
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