产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8243, RRID:AB_2841538.
展开/折叠
hUbc12; NEDD8 carrier protein; NEDD8 conjugating enzyme Ubc12; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBC12_HUMAN; ube2m; ubiquitin carrier protein M; Ubiquitin conjugating enzyme E2 M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-protein ligase M; yeast UBC12 homolog;
抗原和靶标
- P61081 UBC12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61081 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K3 | Acetylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| T20 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| R33 | Methylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| C47 | S-Nitrosylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K61 | Methylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| C65 | S-Nitrosylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| R157 | Methylation | Uniprot | |
| R166 | Methylation | Uniprot | |
| R169 | Methylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| R180 | Methylation | Uniprot | |
| C181 | S-Nitrosylation | Uniprot |
研究背景
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain).
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
限制条款
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