(3)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8506, RRID:AB_2841712.
展开/折叠
ATP 7A; ATP7A; ATP7A_HUMAN; ATPase copper transporting alpha polypeptide; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome); ATPase Cu++ transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1; Copper-transporting ATPase 1; Cu++ transporting P type ATPase; DSMAX; FLJ17790; MC 1; MC1; Menkes disease associated protein; Menkes disease-associated protein; Menkes syndrome; MK; MNK; OHS; OTTHUMP00000062077; SMAX3;
抗原和靶标
Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.
- Q04656 ATP7A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQEATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTATFIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSEVESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVEYDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKCYIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENADEGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEASLVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMFLERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALKHKTANMDVLIVLATTIAFAYSLIILLVAMYERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDIIKVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISRTETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQVGITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTAL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q04656 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S147 | Phosphorylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K227 | Acetylation | Uniprot | |
| K239 | Acetylation | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| Y343 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| Y471 | Phosphorylation | Uniprot | |
| T476 | Phosphorylation | Uniprot | |
| Y517 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| Y594 | Phosphorylation | Uniprot | |
| T601 | Phosphorylation | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| S827 | Phosphorylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| K855 | Ubiquitination | Uniprot | |
| K1037 | Ubiquitination | Uniprot | |
| K1039 | Ubiquitination | Uniprot | |
| K1045 | Ubiquitination | Uniprot | |
| K1060 | Ubiquitination | Uniprot | |
| K1132 | Ubiquitination | Uniprot | |
| T1204 | Phosphorylation | Uniprot | |
| T1212 | Phosphorylation | Uniprot | |
| S1306 | Phosphorylation | Uniprot | |
| K1412 | Ubiquitination | Uniprot | |
| S1424 | Phosphorylation | Uniprot | |
| S1430 | Phosphorylation | Uniprot | |
| S1432 | Phosphorylation | Uniprot | |
| S1435 | Phosphorylation | Uniprot | |
| S1444 | Phosphorylation | Uniprot | |
| S1447 | Phosphorylation | Uniprot | |
| K1449 | Ubiquitination | Uniprot | |
| Y1459 | Phosphorylation | Uniprot | |
| S1460 | Phosphorylation | Uniprot | |
| S1463 | Phosphorylation | Uniprot | |
| S1466 | Phosphorylation | Uniprot | |
| S1469 | Phosphorylation | Uniprot | |
| K1471 | Ubiquitination | Uniprot | |
| S1473 | Phosphorylation | Uniprot | |
| S1476 | Phosphorylation | Uniprot | |
| T1479 | Phosphorylation | Uniprot | |
| S1480 | Phosphorylation | Uniprot | |
| S1486 | Phosphorylation | Uniprot | |
| T1498 | Phosphorylation | Uniprot |
研究背景
May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane (PubMed:9147644). Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels.
Cytoplasm>Cytosol.
Endoplasmic reticulum.
Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.
Monomer. Interacts with PDZD11. Interacts with ATOX1 and COMMD1.
The C-terminal di-leucine, 1487-Leu-Leu-1488, is an endocytic targeting signal which functions in retrieving recycling from the plasma membrane to the TGN. Mutation of the di-leucine signal results in the accumulation of the protein in the plasma membrane.
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
研究领域
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Organismal Systems > Digestive system > Mineral absorption.
文献引用
Application: IHC Species: Human Sample: HCC tissues
Application: WB Species: Human Sample: fibroblasts
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