OPA1 Antibody - #DF8587

>>满百送 进行中>>
>>赠JD卡 进行中>>
(10)   (7)  
产品: OPA1 抗体
货号: DF8587
描述: Rabbit polyclonal antibody to OPA1
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 111kDa, 80-95kDa; 112kD(Calculated).
蛋白号: O60313
RRID: AB_2841791

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
克隆:
Polyclonal
特异性:
OPA1 Antibody detects endogenous levels of total OPA1.
RRID:
AB_2841791
引用格式: Affinity Biosciences Cat# DF8587, RRID:AB_2841791.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Dynamin like 120 kDa protein; Dynamin like 120 kDa protein, mitochondrial; Dynamin-like 120 kDa protein, form S1; FLJ12460; Juvenile kjer type optic atrophy; KIAA0567; KJER type; Large GTP binding protein; largeG; MGM1; Mitochondrial dynamin like 120 kDa protein; Mitochondrial dynamin like GTPase; NPG; NTG; OAK; OPA 1; opa1; OPA1 gene; OPA1_HUMAN; Optic atrophy 1 (autosomal dominant); OPTIC ATROPHY 1; Optic atrophy 1 gene protein; Optic atrophy 1 homolog (human); Optic atrophy protein 1; Optic atrophy protein 1 homolog;

抗原和靶标

免疫原:
Uniprot:

O60313(OPA1_HUMAN) >>Visit HPA database.

基因/基因ID:
OPA1(4976)
表达:
O60313 OPA1_HUMAN:

Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.

序列:
MWRLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRYLILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPDFDKIVESLSLLKDFFTSGSPEETAFRATDRGSESDKHFRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Horse
92
Xenopus
83
Zebrafish
83
Chicken
83
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60313 作为底物

Site PTM Type Enzyme
S21 Phosphorylation
S22 Phosphorylation
S107 Phosphorylation
T114 Phosphorylation
K117 Ubiquitination
K123 Ubiquitination
Y132 Phosphorylation
K154 Ubiquitination
K164 Ubiquitination
K214 Ubiquitination
K228 Acetylation
K228 Ubiquitination
Y268 Phosphorylation
S298 Phosphorylation
K301 Ubiquitination
T302 Phosphorylation
S344 Phosphorylation
S345 Phosphorylation
T351 Phosphorylation
K352 Ubiquitination
S383 Phosphorylation
K387 Ubiquitination
T405 Phosphorylation
S440 Phosphorylation
K474 Ubiquitination
S478 Phosphorylation
K489 Ubiquitination
K527 Ubiquitination
K530 Ubiquitination
K535 Ubiquitination
K568 Ubiquitination
Y582 Phosphorylation
K598 Ubiquitination
S607 Phosphorylation
T612 Phosphorylation
K663 Ubiquitination
S682 Phosphorylation
K699 Ubiquitination
S708 Phosphorylation
K713 Ubiquitination
R733 Methylation
K772 Acetylation
Y808 Phosphorylation
T815 Phosphorylation
T816 Phosphorylation
R824 Methylation
S831 Phosphorylation
K834 Acetylation
K834 Ubiquitination
K911 Ubiquitination
K923 Ubiquitination
K926 Acetylation
T929 Phosphorylation
K931 Acetylation
K940 Ubiquitination
K948 Ubiquitination

研究背景

功能:

Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission. Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes. Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Plays a role in mitochondrial genome maintenance.

Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.

Isoforms that contain the alternative exon 4b (present in isoform 4 and isoform 5) are required for mitochondrial genome maintenance, possibly by anchoring the mitochondrial nucleoids to the inner mitochondrial membrane.

翻译修饰:

PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions.

Cleavage at position S2 is mediated by YME1L. Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ). This motif is present in isoform 2, isoform 3, isoform 4 and isoform 7, but is absent in the displayed isoform 1 and in isoform 5.

细胞定位:

Mitochondrion inner membrane>Single-pass membrane protein. Mitochondrion intermembrane space. Mitochondrion membrane.
Note: Detected at contact sites between endoplamic reticulum and mitochondrion membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.

亚基结构:

Oligomeric complex consisting of membrane-bound and soluble forms of OPA1. Interacts with RCC1L; this interaction is direct. Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms. Binds PARL (By similarity). Interacts with PRELID1.

蛋白家族:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

文献引用

1). Drp1-dependent mitochondrial fission contributes to Cr (VI)-induced mitophagy and hepatotoxicity. Ecotoxicology and Environmental Safety, 2020 (PubMed: 32888618) [IF=6.8]

Application: WB    Species: human    Sample: hepatocytes

Fig. 1.| Cr(VI) induced mitochondrial fission in the hepatocytes. (C) The protein expressions of the five molecules control the fusion/fission process, OPA1, MFN1, MFN2, FIS1, and Drp1 were assayed using western blotting. Mitochondrial protein was also extracted to determine the protein level of Drp1. VDAC1 (32 kDa) was the internal standard for the mitochondrial protein.
2). Valproic acid regulates MIEF1 through MST2-HIPPO to suppress breast cancer growth. Life sciences, 2022 (PubMed: 36126724) [IF=6.1]
3). Ginsenoside CK improves skeletal muscle insulin resistance by activating DRP1/PINK1-mediated mitophagy. Food & Function, 2023 (PubMed: 36562271) [IF=6.1]
4). FGFR2 Mutation p.Cys342Arg Enhances Mitochondrial Metabolism-Mediated Osteogenesis via FGF/FGFR-AMPK-Erk1/2 Axis in Crouzon Syndrome. Cells, 2022 (PubMed: 36231091) [IF=6.0]

Application: WB    Species: Human    Sample:

Figure 7 Mitochondrial dynamics participate in osteogenesis mediated by FGFR/FGFR2-AMPK pathway: (A) Mito-tracker red and mitochondria 2D analysis showed more fragmented or punctate mitochondria with fewer and shorter branches in the MT group, while there were widely reticular mitochondria with longer branches in the WT group. (B) qRT-PCR and Western blot analysis demonstrated that the expressions of mitochondrial-fusion-related factors Mfn2 and Opa1 were downregulated, however, the expression of mitochondrial-fission-related factor Drp1 was upregulated in the MT group compared to the WT group. (C) Mito-tracker red and mitochondria 2D analysis showed a filamentous network of mitochondria with more and longer branches widely spread in the cytoplasm in the siFGFR2 group. (D) qPCR and Western blot analysis showed that the expression of fusion-related genes Mfn2 and Opa1 was increased and the expression of fission-related gene Drp1 was decreased in the siFGFR2 group. (E) After treatment with Compound C, the morphology of the mitochondria which should have tended to split adopted a fused reticular structure with more and longer branches, as shown by Mito-tracker red and mitochondria 2D analysis. (F) Western blot analysis showed the level of MFN2 and OPA1 was increased and then level of DRP1 was decreased, as induced by Compound C. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
5). Low-dose atorvastatin protects skeletal muscle mitochondria in high-fat diet-fed mice with mitochondrial autophagy inhibition and fusion enhancement. European journal of pharmacology, 2023 (PubMed: 37806539) [IF=5.0]

Application: WB    Species: Mouse    Sample:

Fig. 4. Ator treatment regulates expression of the proteins related to mitochondrial quality-control system in the skeletal muscles of mice. (A) Representative immunoblots for PINK1, LC3, PGC-1α, TFAM, and GAPDH protein in indicated skeletal muscles; (B–E) Quantification of PINK1, LC3, PGC-1α, and TFAM by densitometric analysis (n = 12); (F) Representative immunoblots for MFN1, MFN2, OPA1, DRP1, and GAPDH protein in indicated skeletal muscles; (G–J) Quantification of MFN1, MFN2, OPA1, and DRP1 by densitometric analysis (n = 12); (K) Correlation analysis between relative proteins expression (36 samples/variable). All data are shown as Mean ± SEM.
6). Glucose fluctuation accelerates cardiac injury of diabetic mice via sodium-dependent glucose cotransporter 1 (SGLT1). ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 34153296) [IF=3.9]

Application: WB    Species: Mice    Sample: heart

Fig. 7. SGLT1 knockdown promoted mitochondrial fusion and suppressed mitochondrial fission in heart of FDM mice. (A) The expression levels of mitochondrial fusion-related proteins (Mfn1, Mfn2, and Opa1) and fission-related proteins (Drp1 and Fis1) were detected by Western blot analysis. (B) Quantitative analysis of proteins in A was presented. Data were presented as mean ± SD (n = 6), and were compared with one-way ANOVA followed by post hoc Tukey’s test.
7). Knockdown of SGLT1 prevents the apoptosis of cardiomyocytes induced by glucose fluctuation via relieving oxidative stress and mitochondrial dysfunction. Biochemistry and Cell Biology, 2021 (PubMed: 33259229) [IF=2.9]
8). GSDMD induces hepatocyte pyroptosis to trigger alcoholic hepatitis through modulating mitochondrial dysfunction. Cell division, 2024 (PubMed: 38532477) [IF=2.3]
9). M1 Microglia Induced Neuronal Injury on Ischemic Stroke via Mitochondrial Crosstalk between Microglia and Neurons. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 36478988)

Application: WB    Species: Mouse    Sample: M0-BV2 and M1-BV2 cells

Figure 3 Mitochondrial changes in activated microglia (M1). (a) Schematic diagram of intracellular mitochondrial fusion and fission process in microglia after OGD/R. (b) Western blot assay of mitochondrial fusion protein (Opa1 and Mfn1), TOM20, and cytochrome c in mitochondria of M0 and M1 microglia (n = 3). Results are displayed in a form of mean ± SD; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. (c) Western blotting findings of mitochondrial fission protein including MFF, Fis1, Mid49, and Mid51 in mitochondria of M0 and M1 microglia (n = 3). Data presented are mean ± SD; ∗∗P < 0.01 and ∗∗∗P < 0.001. (d) Mitochondrial function in M1 microglia and M0 microglia was investigated by determining ATP (n = 3), mitochondria membrane potential (n = 3), and ROS (n = 3). The relative ratio of intracellular ATP was determined by calculating the ratio of level of ATP in M0-BV2 and M1-BV2 cells to level of ATP in M0-BV2 cells. Results are displayed in a form of mean ± SD. ∗∗P < 0.01 and ∗∗∗P < 0.001. (e) Morphology of intracellular mitochondria in BV2 cells visualized under TEM, scale bar: 1.0 μm.
10). Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34234886)

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.