产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9999, RRID:AB_2843191.
展开/折叠
E2 17kDa; E2 protein; hHR6B; HR6B; RAD6 homolog B; RAD6B; UBC2; UBE2B; UBE2B_HUMAN; Ubiquitin carrier protein B; Ubiquitin conjugating enzyme E2 B; Ubiquitin conjugating enzyme E2B (RAD6 homolog); Ubiquitin conjugating enzyme E2B; Ubiquitin protein ligase B; Ubiquitin-conjugating enzyme E2 17 kDa; Ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B; BHR6A; hHR6A; HR6A; mHR6A; MRXS30; MRXSN; RAD6 homolog A; RAD6A; UBC2; UBCD6; UBE2A; Ube2a ubiquitin-conjugating enzyme E2A; UBE2A_HUMAN; Ubiquitin carrier protein A; Ubiquitin carrier protein; Ubiquitin conjugating enzyme E2 17 kDa; Ubiquitin conjugating enzyme E2 21.5 kDa; Ubiquitin conjugating enzyme E2A (RAD6 homolog); Ubiquitin conjugating enzyme E2A; Ubiquitin protein ligase A; Ubiquitin-conjugating enzyme E2 A; Ubiquitin-protein ligase A;
抗原和靶标
- P63146 UBE2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
- P49459 UBE2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P63146/P49459 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot |
研究背景
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Cell membrane. Nucleus.
Note: In peripheral neurons, expressed both at the plasma membrane and in nuclei.
Interacts with RAD18, UBR2 and WAC.
Belongs to the ubiquitin-conjugating enzyme family.
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.
Interacts with RAD18 and WAC. Interacts with RFPL4A and CCNB1 (By similarity).
Belongs to the ubiquitin-conjugating enzyme family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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