产品: IFIT1 抗体
货号: AF9093
描述: Rabbit polyclonal antibody to IFIT1
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Horse
分子量: 55kDa; 55kD(Calculated).
蛋白号: P09914
RRID: AB_2843284

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   规格 价格 库存
 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(86%)
克隆:
Polyclonal
特异性:
IFIT1 Antibody detects endogenous levels of total IFIT1.
RRID:
AB_2843284
引用格式: Affinity Biosciences Cat# AF9093, RRID:AB_2843284.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C56; G10P1; GARG 16; GARG16; IFI 56; IFI 56K; IFI-56K; IFI56; IFIT-1; Ifit1; IFIT1_HUMAN; IFNAI1; Interferon alpha inducible protein (MW 56kD); Interferon induced 56 kDa protein; Interferon induced protein 56; Interferon inducible mRNA 561; Interferon-induced 56 kDa protein; Interferon-induced protein with tetratricopeptide repeats 1; ISG56; OTTHUMP00000020062; P56; RNM561;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MSTNGDDHQVKDSLEQLRCHFTWELSIDDDEMPDLENRVLDQIEFLDTKYSVGIHNLLAYVKHLKGQNEEALKSLKEAENLMQEEHDNQANVRSLVTWGNFAWMYYHMGRLAEAQTYLDKVENICKKLSNPFRYRMECPEIDCEEGWALLKCGGKNYERAKACFEKVLEVDPENPESSAGYAISAYRLDGFKLATKNHKPFSLLPLRQAVRLNPDNGYIKVLLALKLQDEGQEAEGEKYIEEALANMSSQTYVFRYAAKFYRRKGSVDKALELLKKALQETPTSVLLHHQIGLCYKAQMIQIKEATKGQPRGQNREKLDKMIRSAIFHFESAVEKKPTFEVAHLDLARMYIEAGNHRKAEENFQKLLCMKPVVEETMQDIHFHYGRFQEFQKKSDVNAIIHYLKAIKIEQASLTRDKSINSLKKLVLRKLRRKALDLESLSLLGFVYKLEGNMNEALEYYERALRLAADFENSVRQGP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
86
Bovine
57
Sheep
57
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P09914 作为底物

Site PTM Type Enzyme
K65 Ubiquitination
K73 Acetylation
K73 Ubiquitination
K127 Ubiquitination
K161 Ubiquitination
K166 Ubiquitination
K192 Ubiquitination
K199 Ubiquitination
K226 Ubiquitination
K269 Ubiquitination
K303 Ubiquitination
S324 Phosphorylation
K336 Ubiquitination
K358 Ubiquitination
K392 Ubiquitination
K393 Ubiquitination
Y402 Phosphorylation
K407 Ubiquitination
K417 Ubiquitination
S418 Phosphorylation
K423 Ubiquitination

研究背景

功能:

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.

翻译修饰:

Phosphorylated.

ISGylated.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts (via TPR repeats 4-7) with EEF1A1 (By similarity). Interacts with EIF3C (By similarity). Interacts with IFIT2 and IFIT3. Interacts (via TPR repeats 1-4) with RPL15. Interacts with STING1/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E.

(Microbial infection) Interacts (via TPR repeat 2) with E1 proteins of HPV types 11, 18 and 3; this interaction blocks E1 helicase activity and viral replication.

蛋白家族:

RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats (TPR eddy), which scaffolds unique additional helices that form an RNA binding cleft.

Belongs to the IFIT family.

研究领域

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

文献引用

1). Overexpression of IFIT1 protects against LPS-induced acute lung injury via regulating CCL5-p65NF-κB signaling. International immunopharmacology, 2023 (PubMed: 36446235) [IF=5.6]

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