产品: | IFIT1 抗体 |
货号: | AF9093 |
描述: | Rabbit polyclonal antibody to IFIT1 |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Horse |
分子量: | 55kDa; 55kD(Calculated). |
蛋白号: | P09914 |
RRID: | AB_2843284 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9093, RRID:AB_2843284.
展开/折叠
C56; G10P1; GARG 16; GARG16; IFI 56; IFI 56K; IFI-56K; IFI56; IFIT-1; Ifit1; IFIT1_HUMAN; IFNAI1; Interferon alpha inducible protein (MW 56kD); Interferon induced 56 kDa protein; Interferon induced protein 56; Interferon inducible mRNA 561; Interferon-induced 56 kDa protein; Interferon-induced protein with tetratricopeptide repeats 1; ISG56; OTTHUMP00000020062; P56; RNM561;
抗原和靶标
- P09914 IFIT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTNGDDHQVKDSLEQLRCHFTWELSIDDDEMPDLENRVLDQIEFLDTKYSVGIHNLLAYVKHLKGQNEEALKSLKEAENLMQEEHDNQANVRSLVTWGNFAWMYYHMGRLAEAQTYLDKVENICKKLSNPFRYRMECPEIDCEEGWALLKCGGKNYERAKACFEKVLEVDPENPESSAGYAISAYRLDGFKLATKNHKPFSLLPLRQAVRLNPDNGYIKVLLALKLQDEGQEAEGEKYIEEALANMSSQTYVFRYAAKFYRRKGSVDKALELLKKALQETPTSVLLHHQIGLCYKAQMIQIKEATKGQPRGQNREKLDKMIRSAIFHFESAVEKKPTFEVAHLDLARMYIEAGNHRKAEENFQKLLCMKPVVEETMQDIHFHYGRFQEFQKKSDVNAIIHYLKAIKIEQASLTRDKSINSLKKLVLRKLRRKALDLESLSLLGFVYKLEGNMNEALEYYERALRLAADFENSVRQGP
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09914 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K65 | Ubiquitination | Uniprot | |
K73 | Acetylation | Uniprot | |
K73 | Ubiquitination | Uniprot | |
K127 | Ubiquitination | Uniprot | |
K161 | Ubiquitination | Uniprot | |
K166 | Ubiquitination | Uniprot | |
K192 | Ubiquitination | Uniprot | |
K199 | Ubiquitination | Uniprot | |
K226 | Ubiquitination | Uniprot | |
K269 | Ubiquitination | Uniprot | |
K303 | Ubiquitination | Uniprot | |
S324 | Phosphorylation | Uniprot | |
K336 | Ubiquitination | Uniprot | |
K358 | Ubiquitination | Uniprot | |
K392 | Ubiquitination | Uniprot | |
K393 | Ubiquitination | Uniprot | |
Y402 | Phosphorylation | Uniprot | |
K407 | Ubiquitination | Uniprot | |
K417 | Ubiquitination | Uniprot | |
S418 | Phosphorylation | Uniprot | |
K423 | Ubiquitination | Uniprot |
研究背景
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
Phosphorylated.
ISGylated.
Cytoplasm.
Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts (via TPR repeats 4-7) with EEF1A1 (By similarity). Interacts with EIF3C (By similarity). Interacts with IFIT2 and IFIT3. Interacts (via TPR repeats 1-4) with RPL15. Interacts with STING1/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E.
(Microbial infection) Interacts (via TPR repeat 2) with E1 proteins of HPV types 11, 18 and 3; this interaction blocks E1 helicase activity and viral replication.
RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats (TPR eddy), which scaffolds unique additional helices that form an RNA binding cleft.
Belongs to the IFIT family.
研究领域
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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