产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9219, RRID:AB_2843409.
展开/折叠
alpha regulated poly(A) polymerase; nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I; nuclear speckle targeted PIPK1A regulated poly(A) polymerase; PAP associated domain containing 2; PAPD2; poly(A) polymerase associated domain containing 2; RBM21; RNA binding motif protein 21; RNA-binding motif protein 21; RNA-binding protein 21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; Star-PAP; STARPAP; STPAP_HUMAN; terminal uridylyl transferase 1, U6 snRNA specific; TUT1; TUTase; U6 snRNA specific terminal uridylyltransferase 1; U6 snRNA-specific terminal uridylyltransferase 1; U6 TUTase; U6-TUTase; URLC6;
抗原和靶标
- Q9H6E5 STPAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVDSDVESLPRGGFRCCLCHVTTANRPSLDAHLGGRKHRHLVELRAARKAQGLRSVFVSGFPRDVDSAQLSEYFLAFGPVASVVMDKDKGVFAIVEMGDVGAREAVLSQSQHSLGGHRLRVRPREQKEFQSPASKSPKGAAPDSHQLAKALAEAADVGAQMIKLVGLRELSEAERQLRSLVVALMQEVFTEFFPGCVVHPFGSSINSFDVHGCDLDLFLDLGDLEEPQPVPKAPESPSLDSALASPLDPQALACTPASPPDSQPPASPQDSEALDFETPSSSLAPQTPDSALASETLASPQSLPPASPLLEDREEGDLGKASELAETPKEEKAEGAAMLELVGSILRGCVPGVYRVQTVPSARRPVVKFCHRPSGLHGDVSLSNRLALHNSRFLSLCSELDGRVRPLVYTLRCWAQGRGLSGSGPLLSNYALTLLVIYFLQTRDPPVLPTVSQLTQKAGEGEQVEVDGWDCSFPRDASRLEPSINVEPLSSLLAQFFSCVSCWDLRGSLLSLREGQALPVAGGLPSNLWEGLRLGPLNLQDPFDLSHNVAANVTSRVAGRLQNCCRAAANYCRSLQYQRRSSRGRDWGLLPLLQPSSPSSLLSATPIPLPLAPFTQLTAALVQVFREALGCHIEQATKRTRSEGGGTGESSQGGTSKRLKVDGQKNCCEEGKEEQQGCAGDGGEDRVEEMVIEVGEMVQDWAMQSPGQPGDLPLTTGKHGAPGEEGQPSHAALAERGPKGHEAAQEWSQGEAGKGASLPSSASWRCALWHRVWQGRRRARRRLQQQTKEGAGGGAGTRAGWLATEAQVTQELKGLSGGEERPETEPLLSFVASVSPADRMLTVTPLQDPQGLFPDLHHFLQVFLPQAIRHLK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H6E5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S10 | Phosphorylation | Uniprot | |
| R13 | Methylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| T649 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| K659 | Acetylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| S750 | Phosphorylation | Q13535 (ATR) | Uniprot |
| K790 | Ubiquitination | Uniprot |
研究背景
Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
Phosphorylated by CK1 in the proline-rich (Pro-rich) region.
Nucleus>Nucleolus. Nucleus speckle.
Widely expressed.
Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction.
Belongs to the DNA polymerase type-B-like family.
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