Phospho-MLKL (Ser358) Antibody - #AF7420

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(34)   (27)  
产品: 磷酸化 MLKL (Ser358) 抗体
货号: AF7420
描述: Rabbit polyclonal antibody to Phospho-MLKL (Ser358)
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 54kDa; 54kD(Calculated).
蛋白号: Q8NB16
RRID: AB_2843860

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-MLKL (Ser358) Antibody detects endogenous levels of MLKL only when phosphorylated at Ser358.
RRID:
AB_2843860
引用格式: Affinity Biosciences Cat# AF7420, RRID:AB_2843860.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q8NB16(MLKL_HUMAN) >>Visit HPA database.

基因/基因ID:
MLKL(197259)
序列:
MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

翻译修饰 - Q8NB16 作为底物

Site PTM Type Enzyme
K40 Ubiquitination
K50 Ubiquitination
S52 Phosphorylation
K57 Ubiquitination
T59 Phosphorylation
K66 Ubiquitination
K78 Ubiquitination
S92 Phosphorylation
S106 Phosphorylation
S125 Phosphorylation
S128 Phosphorylation
K157 Ubiquitination
S161 Phosphorylation
K173 Ubiquitination
K183 Ubiquitination
K198 Ubiquitination
K219 Ubiquitination
K230 Ubiquitination
T246 Phosphorylation
K249 Ubiquitination
T302 Phosphorylation
K331 Ubiquitination
S334 Phosphorylation
K354 Acetylation
K354 Ubiquitination
T357 Phosphorylation Q9Y572 (RIPK3)
S358 Phosphorylation Q9Y572 (RIPK3)
T364 Phosphorylation
K372 Ubiquitination
S373 Phosphorylation
T374 Phosphorylation
S393 Phosphorylation
S417 Phosphorylation
S467 Phosphorylation

研究背景

功能:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.

翻译修饰:

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

细胞定位:

Cytoplasm. Cell membrane.
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homooligomer. Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.

蛋白家族:

The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity).

The coiled coil region 2 is responsible for homotrimerization.

Belongs to the protein kinase superfamily.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

文献引用

1). Polystyrene nanoplastics and cadmium co-exposure aggravated cardiomyocyte damage in mice by regulating PANoptosis pathway. Environmental pollution (Barking, Essex : 1987), 2024 (PubMed: 38462200) [IF=8.9]
2). Polysaccharide from Strongylocentrotus nudus eggs regulates intestinal epithelial autophagy through CD36/PI3K-Akt pathway to ameliorate inflammatory bowel disease. International Journal of Biological Macromolecules, 2023 (PubMed: 37327932) [IF=8.2]
3). An integrated network pharmacology approach reveals that Ampelopsis grossedentata improves alcoholic liver disease via TLR4/NF-κB/MLKL pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38981149) [IF=7.9]
4). Pterocephin A, a Novel Triterpenoid Saponin from Pterocephalus hookeri Induced Liver Injury by Activation of Necroptosis. PHYTOMEDICINE, 2021 (PubMed: 33831690) [IF=7.9]
5). Histone deacetylase-mediated silencing of PSTPIP2 expression contributes to aristolochic acid nephropathy-induced PANoptosis. British journal of pharmacology, 2024 (PubMed: 38073114) [IF=7.3]
6). Protective Effect of Calpain Inhibition During Cold Ischemia on Ischemia–reperfusion Injury After Lung Transplantation. Transplantation, 2023 (PubMed: 36648297) [IF=6.2]
7). Cetylpyridinium chloride triggers paraptosis to suppress pancreatic tumor growth via the ERN1-MAP3K5-p38 pathway. iScience, 2024 (PubMed: 39211547) [IF=5.8]
8). Kongensin a attenuates intervertebral disc degeneration by inhibiting TAK1-mediated PANoptosis of nucleus pulposus cells. International immunopharmacology, 2024 (PubMed: 38359662) [IF=5.6]

Application: WB    Species: Rat    Sample: NPCs

Fig. 1. Induction of NPCs PANoptosis by TBHP and alleviation of TBHP-induced NPCs cytotoxicity by KA. a Effects of TBHP on cleaved caspase-3, cleaved caspase-9, Bax, and Bcl2 expression (n = 3). b Effects of TBHP on MLKL and p-MLKL expression (n = 3). c Effects of TBHP on NLRP3, caspase-1, and GSDMD expression (n = 3). d Molecular formula of KA. e Effects of different concentrations of KA on the viability of NPCs for 24 h. f Effects of KA on the viability of NPCs exposed to TBHP for 6 h (n ≥ 3). g, h Representative fluorescence photomicrograph of live and dead cell staining of NPCs (n = 3, scale bar: 50 μm).
9). Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest. International Immunopharmacology, 2021 (PubMed: 33517223) [IF=5.6]
10). Cisatracurium besylate rescues Mycobacterium Tuberculosis-infected macrophages from necroptosis and enhances the bactericidal effect of isoniazid. International Immunopharmacology, 2023 (PubMed: 37182451) [IF=5.6]
加载更多

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.