| 产品: | NDE1 抗体 |
| 货号: | DF12040 |
| 描述: | Rabbit polyclonal antibody to NDE1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 40 kDa; 38kD(Calculated). |
| 蛋白号: | Q9NXR1 |
| RRID: | AB_2844845 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12040, RRID:AB_2844845.
展开/折叠
FLJ20101; HOM TES 87; LIS1 interacting protein NUDE1 rat homolog; LIS1 interacting protein NUDE1; LIS4; NDE 1; NDE1; NDE1_HUMAN; Nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NUDE 1; NudE; NudE nuclear distribution gene E homolog 1 (A. nidulans); NudE nuclear distribution gene E homolog 1; NUDE1;
抗原和靶标
Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum.
- Q9NXR1 NDE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NXR1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T7 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S154 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S211 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S223 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S231 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T240 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K270 | Ubiquitination | Uniprot | |
| Y279 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S307 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
研究背景
Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle. Cleavage furrow.
Note: Localizes to the interphase and S phase centrosome. During mitosis, partially associated with the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis. manner. Also localizes to the cleavage furrow during cytokinesis.
Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum.
Self-associates. Interacts with CNTRL, LIS1, dynein, SLMAP and TCP1 (By similarity). Interacts with CENPF, dynactin, tubulin gamma, PAFAH1B1, PCM1 and PCNT. Interacts with ZNF365.
Belongs to the nudE family.
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