产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12103, RRID:AB_2844908.
展开/折叠
5 methycytoisine methyltransferase; D13Wsu123e; FLJ20303; hTrm4; MISU; Myc induced SUN domain containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family 2 protein; NOL1/NOP2/Sun domain family member 2; NSUN 2; NSUN2; NSUN2_HUMAN; SAKI; Substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine 5 ) methyltransferase; tRNA (cytosine 5 ) methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolo; tRNA methyltransferase 4 homolog;
抗原和靶标
- Q08J23 NSUN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q08J23 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Acetylation | Uniprot | |
| K28 | Sumoylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K43 | Sumoylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K46 | Sumoylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| Y51 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| K88 | Sumoylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| C93 | S-Nitrosylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K97 | Sumoylation | Uniprot | |
| K100 | Sumoylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K111 | Sumoylation | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| K144 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| R256 | Methylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K279 | Sumoylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K286 | Sumoylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K369 | Sumoylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K437 | Sumoylation | Uniprot | |
| K437 | Ubiquitination | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| K445 | Sumoylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T449 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| K464 | Acetylation | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| K508 | Acetylation | Uniprot | |
| K511 | Sumoylation | Uniprot | |
| K511 | Ubiquitination | Uniprot | |
| K516 | Sumoylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| K557 | Sumoylation | Uniprot | |
| Y561 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| R568 | Methylation | Uniprot | |
| K577 | Sumoylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| K579 | Sumoylation | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| K586 | Acetylation | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| Y609 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| K628 | Ubiquitination | Uniprot | |
| K640 | Sumoylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| Y646 | Phosphorylation | Uniprot | |
| K650 | Sumoylation | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| K654 | Sumoylation | Uniprot | |
| K654 | Ubiquitination | Uniprot | |
| K660 | Sumoylation | Uniprot | |
| K660 | Ubiquitination | Uniprot | |
| K692 | Sumoylation | Uniprot | |
| K692 | Ubiquitination | Uniprot | |
| K712 | Ubiquitination | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| T755 | Phosphorylation | Uniprot | |
| C758 | S-Nitrosylation | Uniprot |
研究背景
RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C(5)-methylation of mitochondrial tRNAs. Catalyzes cytosine C(5)-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts. Cytosine C(5)-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling. Also mediates cytosine C(5)-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Cytosine C(5)-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation. May act downstream of Myc to regulate epidermal cell growth and proliferation (By similarity). Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.
Nucleus>Nucleolus. Cytoplasm. Mitochondrion. Cytoplasm>Cytoskeleton>Spindle. Secreted>Extracellular exosome.
Note: Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847). In testis, localizes to the chromatoid body (By similarity).
Expressed in adult and fetal brain and in lymphoblastoid cells.
Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.
Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.
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