xCT Antibody - #DF12509

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产品: xCT 抗体
货号: DF12509
描述: Rabbit polyclonal antibody to xCT
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Horse, Rabbit, Dog
分子量: 35~55 kDa; 55kD(Calculated).
蛋白号: Q9UPY5
RRID: AB_2845314

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(93%), Horse(93%), Rabbit(93%), Dog(93%)
克隆:
Polyclonal
特异性:
xCT Antibody detects endogenous levels of total xCT.
RRID:
AB_2845314
引用格式: Affinity Biosciences Cat# DF12509, RRID:AB_2845314.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Amino acid transport system xc xCT antibody; Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; Calcium channel blocker resistance protein CCBR1 antibody; CCBR1; Cysteine/glutamate transporter antibody; Cystine/glutamate transporter; OTTHUMP00000164578; SLC7A11; Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; solute carrier family 7; Solute carrier family 7 member 11; Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN; xCT; XCT_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q9UPY5(XCT_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC7A11(23657)
序列:
MVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQLKRKVTLLRGVSIIIGTIIGAGIFISPKGVLQNTGSVGMSLTIWTVCGVLSLFGALSYAELGTTIKKSGGHYTYILEVFGPLPAFVRVWVELLIIRPAATAVISLAFGRYILEPFFIQCEIPELAIKLITAVGITVVMVLNSMSVSWSARIQIFLTFCKLTAILIIIVPGVMQLIKGQTQNFKDAFSGRDSSITRLPLAFYYGMYAYAGWFYLNFVTEEVENPEKTIPLAICISMAIVTIGYVLTNVAYFTTINAEELLLSNAVAVTFSERLLGNFSLAVPIFVALSCFGSMNGGVFAVSRLFYVASREGHLPEILSMIHVRKHTPLPAVIVLHPLTMIMLFSGDLDSLLNFLSFARWLFIGLAVAGLIYLRYKCPDMHRPFKVPLFIPALFSFTCLFMVALSLYSDPFSTGIGFVITLTGVPAYYLFIIWDKKPRWFRIMSEKITRTLQIILEVVPEEDKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
93
Horse
93
Dog
93
Rabbit
93
Xenopus
79
Chicken
77
Zebrafish
64
Bovine
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UPY5 作为底物

Site PTM Type Enzyme
K4 Ubiquitination
S8 Phosphorylation
T9 Phosphorylation
S11 Phosphorylation
K12 Sumoylation
K12 Ubiquitination
Y15 Phosphorylation
S26 Phosphorylation
K30 Ubiquitination
K37 Ubiquitination
S51 Phosphorylation
K222 Ubiquitination
S481 Phosphorylation
K483 Ubiquitination

研究背景

功能:

Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.

蛋白家族:

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

文献引用

1). Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.. Biomedicine & Pharmacotherapy (PubMed: 37224754) [IF=7.5]

Application: WB    Species: Mouse    Sample: renal tissues

Fig. 4. The characteristic changes of ferroptosis were rescued by TUDCA treatment in DN mice. The qRT-PCR results of SLC7A11 and TFR-1 in each group (n = 3) (A). The WB results of SLC7A11 and TFR-1 among groups (n = 3) (B - C). Immunohistochemistry and quantitative analysis of SLC7A11 and TFR-1 in NC, DN, and TUDCA group (n = 3) (D - E). The content of iron (F), MDA (G), and GSH (H) in renal tissues (n = 6). Data are expressed as the mean ± SEM.

Application: IHC    Species: Mouse    Sample: renal tissues

Fig. 4. The characteristic changes of ferroptosis were rescued by TUDCA treatment in DN mice. The qRT-PCR results of SLC7A11 and TFR-1 in each group (n = 3) (A). The WB results of SLC7A11 and TFR-1 among groups (n = 3) (B - C). Immunohistochemistry and quantitative analysis of SLC7A11 and TFR-1 in NC, DN, and TUDCA group (n = 3) (D - E). The content of iron (F), MDA (G), and GSH (H) in renal tissues (n = 6). Data are expressed as the mean ± SEM.
2). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy (PubMed: 36379122) [IF=7.5]
3). GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract. Free Radical Biology and Medicine (PubMed: 37156294) [IF=7.4]
4). YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis. Frontiers in Immunology (PubMed: 35979359) [IF=7.3]
5). KLF11 regulates lung adenocarcinoma ferroptosis and chemosensitivity by suppressing GPX4. Communications Biology (PubMed: 37248295) [IF=5.9]

Application: WB    Species: Human    Sample: A549 cells

Fig. 4: Identification of GPX4 as the target molecule of KLF11 to regulate ferroptosis. a Schematic diagram of RNA-seq screening workflow in A549 cell line transfected with KLF11-NC and KLF11-OE. b Volcano plot showing differential expression of genes between the A549-KLF11-NC and KLF11-OE. c Venn plot showing the intersection of predicted targets of KLF11. d, e qRT-PCR results showed differences in RNA levels of three key molecules (GPX4, SLC7A11 and ASCL4) of ferroptosis between the KLF11-NC, KLF11-OE and KLF11-KO of A549 and PC9 cell lines. f, g Western blot results showed differences in protein levels of GPX4, SLC7A11, and ASCL4 of ferroptosis between the KLF11-NC, KLF11-OE, and KLF11-KO of A549 and PC9 cell lines.
6). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular medicine (Cambridge, Mass.) (PubMed: 37875838) [IF=5.7]

Application: WB    Species: Mouse    Sample: kidney tissues

Fig. 2 CX3CL1 deficiency reduced ferroptosis in renal tissues with cisplatin-induced AKI. A–D RNA sequencing analysis. E–H Measurement of Fe2+, GSH/GSSG ratio, MDA, and SOD levels in kidney tissues. I Expression of ferritin heavy chain in kidney tissues, as measured using western blotting. J Representative images displaying the expression of 3-NT, 4-HNE, and GPX4 as determined by immunohistochemistry. Scale bar = 20 μm. K Western blotting analysis of GPX4 and XCT expression profiles. (AKI, acute kidney injury; GSH/GSSG, glutathione/oxidized glutathione; MDA, malondialdehyde; SOD, superoxide dismutase; 3-NT, 3-nitrotyrosine; 4-HNE, 4-Hydroxynonenal; GPX4, glutathione peroxidase 4; XCT, solute carrier family 7 member 11; P value was calculated by one-way analysis of variance and Tukey’s test.
7). Tetrahydroxy stilbene glycoside ameliorates Alzheimer's disease in APP/PS1 mice via glutathione peroxidase related ferroptosis. International Immunopharmacology (PubMed: 34333354) [IF=5.6]

Application: IHC    Species: Mice    Sample: hippocampus tissues

Fig. 7. TSG mediates the key proteins of ferroptosis as GPX4 and xCT. We detected the mouse hippocampus of CA1, CA2, CA3 and DG regions. The ferroptosis related important proteins were detected by immunohistochemistry as (A) GPX4 and (B) xCT. The random grouping situation was WT group, APP/PS1 model group, TSG (60, 120 and 180 mg/kg) groups. Scare bar: 500, 100 and 50 μ m, n = 5/group.

Application: WB    Species: Mice    Sample: glial cells

Fig. 8. TSG regulates the ferroptosis in various glial cells in APP/PS1 and WT mouse brains. Western blotting assay was performed to detect the expression of related proteins. (A) The protein expression levels of GPX4, GPX1, FTH1, xCT, CD98, DMT1, ACSL4, NCOA4, Ferritin Light Chain and Ferritin Heavy Chain were assessed. The levels were normalized to β-actin. (B) GPX4, GFAP and DAPI fluorescence staining co-localization of WT, APP/PS1 and TSG 60 mg/kg group. (C) GPX4, Iba1 and DAPI fluo- rescence staining co-localization of WT, APP/PS1 and TSG 60 mg/kg group. P # < 0.05, P ## < 0.01, P ### < 0.001 vs WT group. P* < 0.05, P ** < 0.01, P *** < 0.001 vs APP/PS1 group. 2×, 100 × magnification, scare bar: 500 and 100 μ m, n = 5/group.
8). Therapeutic Potential of Astragaloside IV Against Adriamycin-Induced Renal Damage in Rats via Ferroptosis. Frontiers in Pharmacology (PubMed: 35370757) [IF=5.6]

Application: WB    Species: rat    Sample: kidney

FIGURE 4 | ASIV attenuated ferroptosis-related indicators in the ADR-reduced renal damage.(D) Western blot for GPx4, SLC7A11, Nrf2, HO-1, p-Pi3K, and p-Akt in the rat kidney.
9). Protective efficacy of Schizandrin B on ameliorating nephrolithiasis via regulating GSK3β/Nrf2 signaling-mediated ferroptosis in vivo and in vitro. International Immunopharmacology (PubMed: 36940552) [IF=5.6]
10). Black phosphorus quantum dots induced ferroptosis in lung cell via increasing lipid peroxidation and iron accumulation. Food and Chemical Toxicology (PubMed: 37481226) [IF=4.3]
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