xCT Antibody - #DF12509

Figure 4 FGF2 reduces I/R-induced ferroptosis in ECs. (A) Heatmap displays the PBS and I/R cohorts' distinctive abundance characteristics. (B) The KEGG database's functional classification of the FGF2-regulated genes. (C) WB assay of GPX4, 4-HNE, and SLC7A11 in skeletal muscle tissues. (D) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (C) with normalised to GAPDH band density. (E) WB assay of GPX4, 4-HNE, and SLC7A11 in skeletal muscle samples. (F) Skeletal muscle slices with GPX4 and CD31, an EC marker, stained. DAPI staining is used to identify the nuclei. Scale bars: 100 μm. (G) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (E) with normalized with respect to GAPDH band density. (H) Quantification of GPX4 and CD31 double-positive cells, the percentages of double positive cells versus total CD31 positive cells are indicated. (I) Content of Fe2+ was plotted as a histogram. (J) A histogram displaying the MDA content. (K) Content of GSH was plotted as a histogram. (L) Sections of skeletal muscle stained with 4-HNE, with DAPI staining identifying the nuclei. Scale bars: 100 μm. Based on the immunofluorescence results in (L), the average 4-HNE optical density is shown in (M). Data are presented as mean ± SD (n = 3-5 per group). Significance: *P < 0.05.