xCT Antibody - #DF12509

Figure 4 FGF2 reduces I/R-induced ferroptosis in ECs. (A) Heatmap displays the PBS and I/R cohorts' distinctive abundance characteristics. (B) The KEGG database's functional classification of the FGF2-regulated genes. (C) WB assay of GPX4, 4-HNE, and SLC7A11 in skeletal muscle tissues. (D) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (C) with normalised to GAPDH band density. (E) WB assay of GPX4, 4-HNE, and SLC7A11 in skeletal muscle samples. (F) Skeletal muscle slices with GPX4 and CD31, an EC marker, stained. DAPI staining is used to identify the nuclei. Scale bars: 100 μm. (G) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (E) with normalized with respect to GAPDH band density. (H) Quantification of GPX4 and CD31 double-positive cells, the percentages of double positive cells versus total CD31 positive cells are indicated. (I) Content of Fe2+ was plotted as a histogram. (J) A histogram displaying the MDA content. (K) Content of GSH was plotted as a histogram. (L) Sections of skeletal muscle stained with 4-HNE, with DAPI staining identifying the nuclei. Scale bars: 100 μm. Based on the immunofluorescence results in (L), the average 4-HNE optical density is shown in (M). Data are presented as mean ± SD (n = 3-5 per group). Significance: *P < 0.05.

Fig. 4. The characteristic changes of ferroptosis were rescued by TUDCA treatment in DN mice. The qRT-PCR results of SLC7A11 and TFR-1 in each group (n = 3) (A). The WB results of SLC7A11 and TFR-1 among groups (n = 3) (B - C). Immunohistochemistry and quantitative analysis of SLC7A11 and TFR-1 in NC, DN, and TUDCA group (n = 3) (D - E). The content of iron (F), MDA (G), and GSH (H) in renal tissues (n = 6). Data are expressed as the mean ± SEM.

Fig. 4. The characteristic changes of ferroptosis were rescued by TUDCA treatment in DN mice. The qRT-PCR results of SLC7A11 and TFR-1 in each group (n = 3) (A). The WB results of SLC7A11 and TFR-1 among groups (n = 3) (B - C). Immunohistochemistry and quantitative analysis of SLC7A11 and TFR-1 in NC, DN, and TUDCA group (n = 3) (D - E). The content of iron (F), MDA (G), and GSH (H) in renal tissues (n = 6). Data are expressed as the mean ± SEM.

Fig. 4. The effect of ellagic acid on arsenic-induced ferroptosis. (A) Transmission electron microscopy images of mitochondrial morphology in the hippocampus (scale bar=2 μm). (B) Western blot analysis of xCT and GPX4 in the hippocampus. (C-D) Relative density analysis of xCT and GPX4 protein bands in the hippocampus. (E) Western blot analysis of xCT and GPX4 in HT22 cells. (F-G) Relative density analysis of xCT and GPX4 protein bands in HT22 cells. Data are presented as mean ± SD (n=3). * significant difference compared to the control group (*P

Fig. 4: Identification of GPX4 as the target molecule of KLF11 to regulate ferroptosis. a Schematic diagram of RNA-seq screening workflow in A549 cell line transfected with KLF11-NC and KLF11-OE. b Volcano plot showing differential expression of genes between the A549-KLF11-NC and KLF11-OE. c Venn plot showing the intersection of predicted targets of KLF11. d, e qRT-PCR results showed differences in RNA levels of three key molecules (GPX4, SLC7A11 and ASCL4) of ferroptosis between the KLF11-NC, KLF11-OE and KLF11-KO of A549 and PC9 cell lines. f, g Western blot results showed differences in protein levels of GPX4, SLC7A11, and ASCL4 of ferroptosis between the KLF11-NC, KLF11-OE, and KLF11-KO of A549 and PC9 cell lines.