(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12740, RRID:AB_2845701.
展开/折叠
DCT 1; dct-1; DCT1; Divalent cation transporter 1; Divalent metal transporter 1; DMT 1; DMT-1; DMT1; FLJ37416; Natural resistance associated macrophage protein 2; Natural resistance-associated macrophage protein 2; NRAM2_HUMAN; NRAMP 2; NRAMP2; OK/SW-cl.20; Slc11a2; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2; Solute carrier family 11 member 2;
抗原和靶标
Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
- P49281 NRAM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKLWAFTGPGFLMSIAYLDPGNIESDLQSGAVAGFKLLWILLLATLVGLLLQRLAARLGVVTGLHLAEVCHRQYPKVPRVILWLMVELAIIGSDMQEVIGSAIAINLLSVGRIPLWGGVLITIADTFVFLFLDKYGLRKLEAFFGFLITIMALTFGYEYVTVKPSQSQVLKGMFVPSCSGCRTPQIEQAVGIVGAVIMPHNMYLHSALVKSRQVNRNNKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFGKTNEQVVEVCTNTSSPHAGLFPKDNSTLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTYSGQFVMEGFLNLKWSRFARVVLTRSIAIIPTLLVAVFQDVEHLTGMNDFLNVLQSLQLPFALIPILTFTSLRPVMSDFANGLGWRIAGGILVLIICSINMYFVVVYVRDLGHVALYVVAAVVSVAYLGFVFYLGWQCLIALGMSFLDCGHTCHLGLTAQPELYLLNTMDADSLVSR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49281 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S21 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot |
研究背景
Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. May serve to import iron into the mitochondria.
Ubiquitinated by WWP2.
N-glycosylated.
Cell membrane>Multi-pass membrane protein. Early endosome.
Endosome membrane>Multi-pass membrane protein. Mitochondrion outer membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Also found in extracellular vesicles different from exosomes.
Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
Forms a complex with NDFIP1 and NEDD4L, in cortical neurons, in response to iron and colbalt exposure; this interaction leads to ubiquitination by NEDD4L and proteasome-dependent degradation. Interacts with NDFIP2. Interacts with COX2 and TOM6 at the outer mitochondrion membrane. Interacts with ARRDC1; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism. Interacts with ARRDC4; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism (By similarity).
Belongs to the NRAMP family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Organismal Systems > Digestive system > Mineral absorption.
文献引用
Application: WB Species: Human Sample: OS cells
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