| 产品: | RIT1 抗体 |
| 货号: | AF0250 |
| 描述: | Rabbit polyclonal antibody to RIT1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 25kDa; 25kD(Calculated). |
| 蛋白号: | Q92963 |
| RRID: | AB_2833425 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0250, RRID:AB_2833425.
展开/折叠
GTP binding protein Roc1; GTP-binding protein Rit1; NS8; Ras like protein expressed in many tissues; Ras like without CAAX 1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1; RIBB; Ric like expressed in many tissues; Ric-like protein without CAAX motif 1; RIT; RIT1; RIT1_HUMAN; ROC1;
抗原和靶标
- Q92963 RIT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSGTRPVGSCCSSPAGLSREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92963 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot |
研究背景
Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation.
Cell membrane.
Expressed in many tissues.
Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state. Interacts with RGL3 (By similarity).
Belongs to the small GTPase superfamily. Ras family.
限制条款
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