(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13487, RRID:AB_2846506.
展开/折叠
Acetyltransferase mec 17 homolog; Acetyltransferase mec-17 homolog; Alpha TAT; Alpha tubulin acetyltransferase 1; Alpha tubulin N acetyltransferase; Alpha-TAT; Alpha-tubulin N-acetyltransferase; ATAT_HUMAN; ATAT1; Chromosome 6 open reading frame 134; Hypothetical protein LOC79969; MEC17; Nbla00487; TAT;
抗原和靶标
- Q5SQI0 ATAT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQETKNRSASEEQALSQDGSGEKPMHTAPPQAPAPPAQSWTVGGDILNARFIRNLQERRSTRPW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q5SQI0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K56 | Methylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K233 | Acetylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| R293 | Methylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| R323 | Methylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K380 | Acetylation | Uniprot |
研究背景
Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.
Autoacetylation strongly increases tubulin acetylation.
Cytoplasm. Membrane>Clathrin-coated pit. Cell junction>Focal adhesion. Cell projection>Axon. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle.
Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.
Belongs to the acetyltransferase ATAT1 family.
文献引用
Application: WB Species: Rat Sample:
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