产品: IKK alpha/ beta 抗体
货号: AF6014
来源: Rabbit
应用: WB, IHC, IF/ICC, ELISA(peptide)
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 85kD; 85kD,87kD(Calculated).
蛋白号: O15111 | O14920
RRID: AB_2834948

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货期: 当天发货

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
IKK alpha/ beta Antibody detects endogenous levels of total IKK alpha/ beta.
RRID:
AB_2834948
引用格式: Affinity Biosciences Cat# AF6014, RRID:AB_2834948.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

chuk; CHUK1; Conserved Helix Loop Helix Ubiquitous Kinase; Conserved helix loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I Kappa B Kinase 1; I Kappa B Kinase Alpha; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK 1; IKK A; IKK a kinase; IKK-A; IKK-alpha; IKK1; IKKA; IKKA_HUMAN; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear Factor Kappa B Inhibitor Kinase Alpha; Nuclear factor NF kappa B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor; TCF-16; TCF16; Transcription factor 16; I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; IMD15; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达特异性:
O15111 IKKA_HUMAN:

Widely expressed.

O14920 IKKB_HUMAN:

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

蛋白描述:
IKK-beta a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.
蛋白序列:
MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE

MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O15111/O14920 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
S6 Phosphorylation
T23 Phosphorylation
K44 Ubiquitination
K106 Ubiquitination
K147 Ubiquitination
K163 Ubiquitination
Y169 Phosphorylation
K171 Ubiquitination
S177 Phosphorylation P54646 (PRKAA2) , PR:000035736 (hMAP3K7/Phos:1) , P17252 (PRKCA) , Q02156 (PRKCE) , O43318 (MAP3K7) , Q99558 (MAP3K14) , Q05513 (PRKCZ) , Q9UHD2 (TBK1)
C179 S-Nitrosylation
T180 Acetylation
T180 Phosphorylation
S181 Phosphorylation Q9UHD2 (TBK1) , P17252 (PRKCA) , O00141 (SGK1) , O15530 (PDPK1) , Q99558 (MAP3K14) , P54646 (PRKAA2) , Q9Y6K9 (IKBKG) , PR:000035736 (hMAP3K7/Phos:1) , O14920 (IKBKB) , O43318 (MAP3K7) , Q05513 (PRKCZ)
Y188 Phosphorylation P12931 (SRC) , P07948 (LYN)
Y199 Phosphorylation P12931 (SRC) , P07948 (LYN)
S239 Phosphorylation
S246 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
S258 Phosphorylation
Y261 Phosphorylation
S267 Phosphorylation
K301 Ubiquitination
T324 Phosphorylation
Y325 Phosphorylation
S332 Phosphorylation
S335 Phosphorylation
K337 Ubiquitination
T344 Phosphorylation
K365 Ubiquitination
T368 Phosphorylation
S393 Phosphorylation
T396 Phosphorylation
Y397 Phosphorylation
T399 Phosphorylation
S402 Phosphorylation
S409 Phosphorylation
S411 Phosphorylation
K418 Ubiquitination
K467 Ubiquitination
S471 Phosphorylation
S474 Phosphorylation
S476 Phosphorylation
T488 Phosphorylation
S489 Phosphorylation
S507 Phosphorylation
K509 Ubiquitination
S550 Phosphorylation
K555 Ubiquitination
T559 Phosphorylation
T583 Phosphorylation
S587 Phosphorylation
S600 Phosphorylation
K603 Ubiquitination
Y609 Phosphorylation
T610 Phosphorylation
K628 Ubiquitination
S634 Phosphorylation
K652 Ubiquitination
K659 Acetylation
K664 Ubiquitination
S670 Phosphorylation O14920 (IKBKB)
S672 Phosphorylation O14920 (IKBKB)
S675 Phosphorylation O14920 (IKBKB)
S679 Phosphorylation O14920 (IKBKB)
S682 Phosphorylation O14920 (IKBKB)
S689 Phosphorylation O14920 (IKBKB)
S692 Phosphorylation O14920 (IKBKB)
T693 Phosphorylation
S695 Phosphorylation O14920 (IKBKB)
S697 Phosphorylation O14920 (IKBKB)
K703 Ubiquitination
S705 Phosphorylation O14920 (IKBKB)
S733 O-Glycosylation
S733 Phosphorylation O14920 (IKBKB) , P53350 (PLK1)
T735 Phosphorylation
S740 Phosphorylation O14920 (IKBKB) , P53350 (PLK1)
S750 Phosphorylation O14920 (IKBKB) , P53350 (PLK1)
S756 Phosphorylation O14920 (IKBKB)
Site PTM Type Enzyme
T23 Phosphorylation Q9Y243 (AKT3) , O00141 (SGK1) , P31749 (AKT1) , P31751 (AKT2)
K44 Ubiquitination
K53 Ubiquitination
K67 Ubiquitination
K105 Ubiquitination
K109 Ubiquitination
K117 Ubiquitination
S119 Acetylation
K139 Ubiquitination
K146 Ubiquitination
K158 Ubiquitination
Y168 Phosphorylation
S176 Phosphorylation Q04759 (PRKCQ) , Q99558 (MAP3K14)
T179 Acetylation
S180 Phosphorylation Q04759 (PRKCQ) , Q99558 (MAP3K14)
K296 Ubiquitination
K322 Acetylation
K366 Ubiquitination
K391 Ubiquitination
K415 Ubiquitination
K438 Acetylation
T463 Phosphorylation
S473 Phosphorylation Q13315 (ATM)
Y500 Phosphorylation
K506 Ubiquitination
S536 Phosphorylation
K569 Ubiquitination
K572 Ubiquitination
S576 Phosphorylation
T593 Phosphorylation
K602 Ubiquitination
K615 Ubiquitination
K617 Ubiquitination
K624 Ubiquitination
T722 Acetylation

翻译修饰 - O15111/O14920 作为激酶

Substrate Site Source
O14920-1 (IKBKB) C179 Uniprot
O14920 (IKBKB) S181 Uniprot
O14920 (IKBKB) S670 Uniprot
O14920 (IKBKB) S672 Uniprot
O14920 (IKBKB) S675 Uniprot
O14920-1 (IKBKB) S679 Uniprot
O14920 (IKBKB) S682 Uniprot
O14920 (IKBKB) S689 Uniprot
O14920-1 (IKBKB) S692 Uniprot
O14920 (IKBKB) S695 Uniprot
O14920-1 (IKBKB) S697 Uniprot
O14920-1 (IKBKB) S705 Uniprot
O14920-1 (IKBKB) S733 Uniprot
O14920 (IKBKB) S740 Uniprot
O14920-1 (IKBKB) S750 Uniprot
O14920 (IKBKB) S756 Uniprot
O43524 (FOXO3) S644 Uniprot
O95999 (BCL10) S134 Uniprot
P01100 (FOS) S308 Uniprot
P04637 (TP53) S362 Uniprot
P04637 (TP53) S366 Uniprot
P19438 (TNFRSF1A) S381 Uniprot
P19838 (NFKB1) S923 Uniprot
P19838-2 (NFKB1) S924 Uniprot
P19838 (NFKB1) S927 Uniprot
P19838-2 (NFKB1) S928 Uniprot
P19838 (NFKB1) S932 Uniprot
P23396 (RPS3) S209 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P31946 (YWHAB) S132 Uniprot
P35568 (IRS1) S268 Uniprot
P35568 (IRS1) S270 Uniprot
P35568 (IRS1) S272 Uniprot
P35568 (IRS1) S274 Uniprot
P35568 (IRS1) S307 Uniprot
P35568 (IRS1) S312 Uniprot
P35568 (IRS1) S341 Uniprot
P35568 (IRS1) S345 Uniprot
P35568 (IRS1) S527 Uniprot
P35568 (IRS1) S531 Uniprot
P42771 (CDKN2A) S8 Uniprot
Q01201 (RELB) S472 Uniprot
Q04206 (RELA) S468 Uniprot
Q04206 (RELA) S536 Uniprot
Q04864 (REL) S557 Uniprot
Q13568 (IRF5) S446 Uniprot
Q14164 (IKBKE) S172 Uniprot
Q14653 (IRF3) S396 Uniprot
Q14653 (IRF3) S398 Uniprot
Q14653 (IRF3) S402 Uniprot
Q14653 (IRF3) T404 Uniprot
Q14653 (IRF3) S405 Uniprot
Q15653 (NFKBIB) S19 Uniprot
Q15653-1 (NFKBIB) S23 Uniprot
Q16875 (PFKFB3) S269 Uniprot
Q5S007 (LRRK2) S910 Uniprot
Q5S007 (LRRK2) S935 Uniprot
Q92574 (TSC1) S487 Uniprot
Q92574 (TSC1) S511 Uniprot
Q96CV9 (OPTN) S513 Uniprot
Q99704 (DOK1) S439 Uniprot
Q99704 (DOK1) S443 Uniprot
Q99704 (DOK1) S446 Uniprot
Q99704 (DOK1) S450 Uniprot
Q9BXH1 (BBC3) S10 Uniprot
Q9H3D4 (TP63) S43 Uniprot
Q9H3D4 (TP63) S51 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9NQC7 (CYLD) S422 Uniprot
Q9NQC7 (CYLD) S432 Uniprot
Q9NQC7 (CYLD) S436 Uniprot
Q9NQC7 (CYLD) S439 Uniprot
Q9NQC7 (CYLD) S441 Uniprot
Q9NQC7 (CYLD) S444 Uniprot
Q9Y4G8 (RAPGEF2) S1254 Uniprot
Q9Y6K9-1 (IKBKG) S31 Uniprot
Q9Y6K9 (IKBKG) S43 Uniprot
Q9Y6K9 (IKBKG) S68 Uniprot
Q9Y6K9 (IKBKG) S85 Uniprot
Q9Y6K9-1 (IKBKG) S376 Uniprot
Q9Y6Q9-5 (NCOA3) S857 Uniprot
Substrate Site Source
O14920-1 (IKBKB) S177 Uniprot
O14920-1 (IKBKB) S181 Uniprot
O43524 (FOXO3) S644 Uniprot
O75925-1 (PIAS1) S90 Uniprot
P03372 (ESR1) S118 Uniprot
P19838-2 (NFKB1) S922 Uniprot
P19838 (NFKB1) S923 Uniprot
P19838-2 (NFKB1) S924 Uniprot
P19838 (NFKB1) S927 Uniprot
P19838-2 (NFKB1) S928 Uniprot
P19838 (NFKB1) T931 Uniprot
P19838 (NFKB1) S932 Uniprot
P19838-2 (NFKB1) T932 Uniprot
P19838-2 (NFKB1) S933 Uniprot
P24385 (CCND1) T286 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P35222 (CTNNB1) S33 Uniprot
P35222 (CTNNB1) S37 Uniprot
P35222 (CTNNB1) T41 Uniprot
P35222 (CTNNB1) S45 Uniprot
P42345 (MTOR) S1415 Uniprot
P84243 (H3F3B) S11 Uniprot
Q00653 (NFKB2) S99 Uniprot
Q00653 (NFKB2) S108 Uniprot
Q00653 (NFKB2) S115 Uniprot
Q00653 (NFKB2) S123 Uniprot
Q00653 (NFKB2) S872 Uniprot
Q01094 (E2F1) S403 Uniprot
Q01201 (RELB) S472 Uniprot
Q04206 (RELA) S536 Uniprot
Q04864 (REL) S557 Uniprot
Q14164 (IKBKE) S172 Uniprot
Q15653-1 (NFKBIB) S19 Uniprot
Q15653-1 (NFKBIB) S23 Uniprot
Q15717 (ELAVL1) S304 Uniprot
Q5S007 (LRRK2) S910 Uniprot
Q5S007 (LRRK2) S935 Uniprot
Q86VP1-2 (TAX1BP1) S593 Uniprot
Q86VP1-2 (TAX1BP1) S624 Uniprot
Q86VP1 (TAX1BP1) S666 Uniprot
Q92793 (CREBBP) S1382 Uniprot
Q92793 (CREBBP) S1386 Uniprot
Q99558 (MAP3K14) T559 Uniprot
Q9BUZ4 (TRAF4) S426 Uniprot
Q9BXH1 (BBC3) S10 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9NQC7 (CYLD) S422 Uniprot
Q9NQC7 (CYLD) S432 Uniprot
Q9NQC7 (CYLD) S436 Uniprot
Q9NQC7 (CYLD) S439 Uniprot
Q9NQC7 (CYLD) S441 Uniprot
Q9NQC7 (CYLD) S444 Uniprot
Q9Y261 (FOXA2) S107 Uniprot
Q9Y261 (FOXA2) S111 Uniprot
Q9Y6Q9-5 (NCOA3) S857 Uniprot

研究背景

功能:

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity).

翻译修饰:

Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.

(Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

细胞定位:

Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65 (By similarity). Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14. Directly interacts with TRPC4AP (By similarity). May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1. Interacts with ARRB1 and ARRB2. Interacts with NLRC5; prevents CHUK phosphorylation and kinase activity. Interacts with PIAS1; this interaction induces PIAS1 phosphorylation. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner. Interacts with FOXO3. Interacts with IFIT5; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation. Interacts with LRRC14. Interacts with SASH1.

(Microbial infection) Interacts with InlC of Listeria monocytogenes.

蛋白家族:

The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

功能:

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity).

翻译修饰:

Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B.

(Microbial infection) Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.

Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to 'Ser-163' does not serve as a monoubiquitination site. According to ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.

(Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia yopJ.

Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B.

细胞定位:

Cytoplasm. Nucleus. Membrane raft.
Note: Colocalized with DPP4 in membrane rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

亚基结构:

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65 (By similarity). Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6 (By similarity). May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with TRIM21. Interacts with NLRC5; prevents IKBKB phosphorylation and kinase activity. Interacts with PDPK1. Interacts with EIF2AK2/PKR. The phosphorylated form interacts with PPM1A and PPM1B. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner. Interacts with IKBKE. Interacts with NAA10, leading to NAA10 degradation. Interacts with FOXO3. Interacts with AKAP13. Interacts with IFIT5; the interaction synergizes the recruitment of IKK to MAP3K7 and enhances IKK phosphorylation. Interacts with LRRC14; disrupts IKBKB-IKBKG interaction preventing I-kappa-B-kinase (IKK) core complex formation and leading to a decrease of IKBKB phosphorylation and NF-kappaB activation. Interacts with SASH1. Interacts with ARFIP2.

(Microbial infection) Interacts with Yersinia yopJ.

(Microbial infection) Interacts with vaccinia virus protein B14.

蛋白家族:

The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Gao X et al. Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from Ganoderma leucocontextum fruiting bodies in vitro and in vivo. Food Chem X 2022 Apr 30;14:100321. (PubMed: 35571333) [IF=9.231]

Application: WB    Species: mouse    Sample: RAW264.7 macrophages

Fig. 4. |Effects of GLP-1 on the expression levels of p-IKKα/β, p-IκBα, IκBα, p-NF-κB p65, and cytoplasmic and nuclear NF-κB p65 in RAW264.7 macrophages (A). Effects of GLP-1 on the expression levels of p-JNK, p-ERK, p-p38, and p-Akt in RAW264.7 macrophages (B).

2). Zhang J et al. Loganin alleviates sepsis-induced acute lung injury by regulating macrophage polarization and inhibiting NLRP3 inflammasome activation. Int Immunopharmacol 2021 Jun;95:107529. (PubMed: 33744777) [IF=5.714]

Application: WB    Species: mice    Sample: Raw 264.7 cells

Fig. 6. Macrophage polarization induced by loganin maybe associated with the ERK and NF-κB pathway. Raw 264.7 cells were subjected to 5 μM, 10 μM, 20 μM loganin or 10 μM SCH772984 (an ERK inhibitor) or 10 μM JSH-23 (an NF-κB inhibitor) in the presence of 100 ng/ml LPS for 48 h. (A, B) The mRNA and protein levels of CD86 and iNOS (M1-macrophages markers) in LPSactivated Raw 264.7 cells were evaluated by RT-PCR and western blotting. (C, D) The mRNA and protein levels of CD206 and Arg-1 (M2-macrophages markers) in LPS-activated Raw 264.7 cells were assessed by RT-PCR and western blotting. Data were shown as means ± SD. n = 3. *p < 0.05 vs. control. #p < 0.05 vs. LPS. ERK, extracellular signal-regulated kinase; NF-κB, nuclear factor-kappa B; iNOS, inducible NO synthase; Arg-1, arginase-1; LPS, lipopolysaccharide.

3). Ma F et al. Qi-dan-di-huang decoction alleviates diabetic nephropathy by inhibiting the NF-kappaB pathway. Front Biosci (Landmark Ed) 2019 Jun 1;24:1477-1486. (PubMed: 31136992) [IF=4.009]

Application: WB    Species: rat    Sample: kidneys

Figure 4. |Effect of QDDH decoction on the NF-κB signaling pathway in DN rats. Western blot was carried out to evaluate the levels of phospho-p65, p65, p-IκBα, IκBα, NF-κB p105/p50, IκBβ, and Iκκα/β in the Con group, DN group, Fen group, and QDDH decoction group. Comparison with the Con group, *P<0.05; comparison with the DN group, #P<0.05.

4). Hu T et al. Zanthoxylum nitidum extract attenuates BMP-2-induced inflammation and hyperpermeability. Biosci Rep 2020 Oct 30;40(10):BSR20201098. (PubMed: 33030503) [IF=3.976]

Application: WB    Species: human    Sample: HUVECs

Figure 2. |BMP-2 promoted the NF-κB signal pathway in HUVECs(A–C) p-IκB and p-p65 protein levels in HUVECs were determined (n=5).

5). Song L et al. Schisandrin ameliorates cognitive deficits, endoplasmic reticulum stress and neuroinflammation in STZ-induced Alzheimer's disease rats. Exp Anim 2020 Apr 24 (PubMed: 32336744) [IF=1.798]

Application: WB    Species: rat    Sample: brain

Figure 5| Schisandrin inhibits the NF-κB signaling in the brain of rats. The protein levels of IκBα and p-IκBα (A); IKKα/β and p- IKKα/β (B) were analyzed by western-blot.

6). Meng S et al. MiR-363-3p/S1PR1 axis inhibits sepsis-induced acute lung injury via the inactivation of NF-κB signaling. Exp Anim 2022 Feb 16. (PubMed: 35173110) [IF=1.798]

Application: WB    Species: mouse    Sample: lung

Fig. 6. |Knockdown of miR-363-3p blocks the NF-κB activation by targeting S1PR1.(c) Relative protein levels of NF-κB p65 (cytoplasm), NF-κB p65 (nucleus), p-IKK Ser180/Ser181, IKK, p-IκB-α Ser32/36, and IκB-α in lung tissues of mice in different groups.

Application: WB    Species: Mice    Sample: lung tissues

Fig. 6. Knockdown of miR-363-3p blocks the NF-κB activation by targeting S1PR1. (a) Relative mRNA levels of S1pr1 in lung tissues of mice in different groups. (b) Relative protein levels of S1PR1 in lung tissues of mice in different groups. (c) Relative protein levels of NF-κB p65 (cytoplasm), NF-κB p65 (nucleus), p-IKK Ser180/Ser181, IKK, p-IκB-α Ser32/36, and IκB-α in lung tissues of mice in different groups. (d) Representative images of dual immunofluorescence staining with S1PR1 and F4/80 in lung tissues of mice in different groups. (e) A luciferase reporter assay was used to measure the relative luciferase activities of S1PR1-3’-UTR wt and mut in RAW264.7 cells. Results were expressed as means ± SD, *P<0.05.

7). Polysaccharide of Atractylodes Macrocephala Koidz (PAMK) Enhance the Immunomodulatory Activity of RAW264. 7 Through TLR4/MyD88/NF-κB Pathway.

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