| 产品: | 磷酸化 Anillin (Ser635) 抗体 |
| 货号: | AF3533 |
| 描述: | Rabbit polyclonal antibody to Phospho-Anillin (Ser635) |
| 应用: | IHC |
| 反应: | Human, Rat |
| 分子量: | 120~170kD; 124kD(Calculated). |
| 蛋白号: | Q9NQW6 |
| RRID: | AB_2846847 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3533, RRID:AB_2846847.
展开/折叠
Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Actin-binding protein anillin; Anillin actin binding protein; anln; ANLN_HUMAN; DKFZp779A055; FSGS8; Scra; Scraps; Scraps homolog;
抗原和靶标
A synthesized peptide derived from human Anillin around the phosphorylation site of Ser635.
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
- Q9NQW6 ANLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVESTSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKAASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLNKALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSAEKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKVEQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLDEKNNAFPCQVNIKQKMQELNNEINMQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATGKRTLLIDELNKLKNEGPQRKNKASPQSEFMPSKGSVTLSEIRLPLKADFVCSTVQKPDAANYYYLIILKAGAENMVATPLASTSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDPSGLDKKKKTSKSKAITPKRLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSLSSVGNTKFVLDKVPFLSSLEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTYPDDEKRKNPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQREDDRETLVSQCRDTLCVTKNWLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP
翻译修饰 - Q9NQW6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| C71 | S-Nitrosylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| K254 | Sumoylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T300 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| C309 | S-Nitrosylation | Uniprot | |
| T320 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| K371 | Acetylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| K413 | Sumoylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| K428 | Methylation | Uniprot | |
| K428 | Sumoylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| T472 | Phosphorylation | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| T483 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| T504 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| T523 | Phosphorylation | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| T628 | Phosphorylation | Uniprot | |
| K634 | Ubiquitination | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| Y671 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| Y676 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| K682 | Ubiquitination | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| K706 | Ubiquitination | Uniprot | |
| K717 | Ubiquitination | Uniprot | |
| K719 | Ubiquitination | Uniprot | |
| T767 | Phosphorylation | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| T771 | Phosphorylation | Uniprot | |
| K779 | Ubiquitination | Uniprot | |
| K790 | Ubiquitination | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| S913 | Phosphorylation | Uniprot | |
| S918 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | Uniprot | |
| K956 | Acetylation | Uniprot | |
| T1079 | Phosphorylation | Uniprot | |
| K1092 | Sumoylation | Uniprot | |
| K1092 | Ubiquitination | Uniprot | |
| K1101 | Ubiquitination | Uniprot | |
| Y1118 | Phosphorylation | Uniprot |
研究背景
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.
Nucleus. Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Cell projection>Bleb.
Note: Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus. Expression is significantly increased in renal biopsy specimens from idiopathic FSGS. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.
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