产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6805, RRID:AB_2847528.
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AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; map2k3; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; protein kinase, mitogen-activated, kinase 3; SAPK kinase 2; SAPKK 2; SAPKK2; Stress activated protein kinase kinase 2; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK 6; MAPKK6; MEK 6; MEK6; Mitogen Activated Protein Kinase Kinase 6; MKK 6; MKK6; MP2K6_HUMAN; PRKMK6; Protein kinase mitogen activated kinase 6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 3; SAPKK-3; SAPKK3; SKK3; Stress-activated protein kinase kinase 3;
抗原和靶标
A synthesized peptide derived from human MKK3/MKK6.
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
P52564 MP2K6_HUMAN:Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
- P46734 MP2K3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS
- P52564 MP2K6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD
翻译修饰 - P46734/P52564 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Q99759 (MAP3K3) , P41279 (MAP3K8) , Q9UL54 (TAOK2) , Q16584 (MAP3K11) , Q7L7X3 (TAOK1) , Q99683 (MAP3K5) , P46734 (MAP2K3) | Uniprot |
| T222 | Phosphorylation | Q16584 (MAP3K11) , P46734 (MAP2K3) | Uniprot |
| Y230 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| K279 | Sumoylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K340 | Ubiquitination | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Acetylation | Uniprot | |
| K8 | Acetylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K82 | Acetylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K141 | Methylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| S207 | Acetylation | Uniprot | |
| S207 | Phosphorylation | P52564 (MAP2K6) , Q99683 (MAP3K5) , O43318 (MAP3K7) , P41279 (MAP3K8) , Q9UL54 (TAOK2) | Uniprot |
| T211 | Acetylation | Uniprot | |
| T211 | Phosphorylation | O43318 (MAP3K7) , Q13233 (MAP3K1) , Q9UL54 (TAOK2) | Uniprot |
| Y219 | Phosphorylation | P52564 (MAP2K6) | Uniprot |
| K232 | Ubiquitination | Uniprot | |
| K268 | Sumoylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot |
翻译修饰 - P46734/P52564 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O15264 (MAPK13) | T180 | Uniprot |
| O15264 (MAPK13) | Y182 | Uniprot |
| P46734 (MAP2K3) | S218 | Uniprot |
| P46734 (MAP2K3) | T222 | Uniprot |
| P53778 (MAPK12) | T183 | Uniprot |
| P53778 (MAPK12) | Y185 | Uniprot |
| Q16539 (MAPK14) | T180 | Uniprot |
| Q16539 (MAPK14) | Y182 | Uniprot |
| Substrate | Site | Source |
|---|---|---|
| O15264-1 (MAPK13) | T180 | Uniprot |
| O15264 (MAPK13) | Y182 | Uniprot |
| P52564 (MAP2K6) | S207 | Uniprot |
| P52564 (MAP2K6) | Y219 | Uniprot |
| P53778 (MAPK12) | T183 | Uniprot |
| P53778 (MAPK12) | Y185 | Uniprot |
| Q14765 (STAT4) | S721 | Uniprot |
| Q15759 (MAPK11) | T180 | Uniprot |
| Q15759 (MAPK11) | Y182 | Uniprot |
| Q16539 (MAPK14) | T180 | Uniprot |
| Q16539-4 (MAPK14) | Y182 | Uniprot |
| Q9NQU5 (PAK6) | T564 | Uniprot |
| Q9NQU5 (PAK6) | Y566 | Uniprot |
| Q9ULV5 (HSF4) | T471 | Uniprot |
研究背景
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK. Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2 (By similarity). Interacts with ARRB1. Interacts with Yersinia yopJ.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton.
Note: Binds to microtubules.
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Dimer. Interacts with Yersinia yopJ. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity). Interacts (via its DVD domain) with MAP3Ks activators like MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2 (By similarity). Interacts with DCTN1. Interacts with EIF2AK2/PKR.
The DVD domain (residues 311-334) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation.
The D domain (residues 4-19) contains a conserved docking site and is required for the binding to MAPK substrates.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
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