产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0287, RRID:AB_2833455.
展开/折叠
Anthracycline associated resistance ARX; Anthracycline-associated resistance ARX; ARX; FLJ13058; HRIHFB2115; SAE 2; SAE2; SAE2_HUMAN; SUMO 1 activating enzyme subunit 2; SUMO activating enzyme subunit 2; SUMO-activating enzyme subunit 2; UBA2; UBA2 ubiquitin activating enzyme E1 homolog; Ubiquitin like 1 activating enzyme E1B; Ubiquitin like modifier activating enzyme 2; Ubiquitin-like 1-activating enzyme E1B; UBLE1B;
抗原和靶标
- Q9UBT2 SAE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UBT2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K65 | Ubiquitination | Uniprot | |
| K77 | Acetylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| K164 | Sumoylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K190 | Sumoylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| R210 | Methylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K236 | Acetylation | Uniprot | |
| K236 | Sumoylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Sumoylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K257 | Sumoylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K271 | Acetylation | Uniprot | |
| K271 | Methylation | Uniprot | |
| K271 | Sumoylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K275 | Sumoylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K316 | Sumoylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K324 | Acetylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| K336 | Acetylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K371 | Sumoylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| K420 | Sumoylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| K540 | Sumoylation | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| T578 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K611 | Sumoylation | Uniprot | |
| K613 | Sumoylation | Uniprot | |
| K617 | Acetylation | Uniprot | |
| K617 | Sumoylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| K623 | Acetylation | Uniprot | |
| K623 | Sumoylation | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| K630 | Sumoylation | Uniprot |
研究背景
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.
Cytoplasm. Nucleus.
Note: Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention.
Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.
Belongs to the ubiquitin-activating E1 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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