| 产品: | PHF1 抗体 |
| 货号: | AF0324 |
| 描述: | Rabbit polyclonal antibody to PHF1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Sheep, Rabbit |
| 分子量: | 62kDa; 62kD(Calculated). |
| 蛋白号: | O43189 |
| RRID: | AB_2833487 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0324, RRID:AB_2833487.
展开/折叠
hPCl1; MTF2 L2; MTF2L 2; MTF2L2; PCL 1; PCL1; PHD finger protein 1; PHD finger protein1; PHF 1; PHF 2; Phf1; PHF1_HUMAN; PHF2; Plc 1; Plc1; Polycomb like protein 1; Polycomb like protein1; Polycomb-like protein 1; Protein PHF1; T complex testis expressed 3; T complex testis expressed3; Tctex 3; Tctex3;
抗原和靶标
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
- O43189 PHF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQPPRLSRSGASSLWDPASPAPTSGPRPRLWEGQDVLARWTDGLLYLGTIKKVDSAREVCLVQFEDDSQFLVLWKDISPAALPGEELLCCVCRSETVVPGNRLVSCEKCRHAYHQDCHVPRAPAPGEGEGTSWVCRQCVFAIATKRGGALKKGPYARAMLGMKLSLPYGLKGLDWDAGHLSNRQQSYCYCGGPGEWNLKMLQCRSCLQWFHEACTQCLSKPLLYGDRFYEFECCVCRGGPEKVRRLQLRWVDVAHLVLYHLSVCCKKKYFDFDREILPFTSENWDSLLLGELSDTPKGERSSRLLSALNSHKDRFISGREIKKRKCLFGLHARMPPPVEPPTGDGALTSFPSGQGPGGGVSRPLGKRRRPEPEPLRRRQKGKVEELGPPSAVRNQPEPQEQRERAHLQRALQASVSPPSPSPNQSYQGSSGYNFRPTDARCLPSSPIRMFASFHPSASTAGTSGDSGPPDRSPLELHIGFPTDIPKSAPHSMTASSSSVSSPSPGLPRRSAPPSPLCRSLSPGTGGGVRGGVGYLSRGDPVRVLARRVRPDGSVQYLVEWGGGGIF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43189 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| R530 | Methylation | Uniprot |
研究背景
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 lociand. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Co-localizes with NEK6 in the centrosome.
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
Interacts with CHMP1 (By similarity). Associated component of the PRC2 complex. Interacts with p53/TP53.
The Tudor domain recognizes and binds H3K36me3 (PubMed:23228662, PubMed:23273982 and PubMed:23142980).
Belongs to the Polycomblike family.
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